Department of Biomedical Engineering, Nation Taiwan University, Taipei,
J Acoust Soc Am. 2020 Oct;148(4):EL314. doi: 10.1121/10.0002138.
A cellular stimulation device with a pressurized chamber is developed to investigate the effect of ultrasound and pressure fluctuation on nucleus pulposus (NP) cells. The pressurized chamber is designed to emulate the in vivo environment of intervertebral discs, which are under dynamic pressure, and to emulate impact during sports and exercise. Both hydrostatic pressure and ultrasound stimulation increase phosphorylation of ERK (pERK) in NP cells, and promote its translocation into nucleus. This increase in pERK levels might be activated through calcium signaling pathways as intracellular calcium in NP cells was strongly elevated by pressure changes.
一种带有加压室的细胞刺激装置被开发出来，用于研究超声和压力波动对髓核（NP）细胞的影响。加压室的设计旨在模拟椎间盘的体内环境，即承受动态压力，并模拟运动和锻炼时的冲击。液压压力和超声刺激均能增加 NP 细胞中 ERK 的磷酸化（pERK），并促进其向核内转位。这种 pERK 水平的增加可能是通过钙信号通路激活的，因为 NP 细胞中的细胞内钙因压力变化而强烈升高。
