Department of Pediatric Dentistry, Hadassah School of Dental Medicine, Hebrew University, Jerusalem, Israel.
Institute of Gastroenterology, Nutrition and Liver Diseases, Schneider Children's Medical Center of Israel, Petach Tikva, and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
Clin Oral Investig. 2021 May;25(5):2993-2998. doi: 10.1007/s00784-020-03619-4. Epub 2020 Nov 2.
The aims of this study were to compare the salivary cytokine profile, as a potential replacement for blood tests, in liver-transplanted children to that of a control group of healthy children, and to correlate the values of commonly tested laboratory blood tests to those of published blood values.
Liver-transplanted children, and a control group of healthy children of the same sex and age distribution, were recruited for the study. Saliva was collected at the same appointment for routine blood tests for the liver-transplanted children. Saliva was also collected from a control group of healthy children with similar age and sex distributions. Normal healthy blood values were extracted from the literature, for comparison. Cytokine levels in the saliva were quantified with ELISA. The analysis compared serum and saliva values between liver-transplanted and healthy children. In the serum, the values of albumin, GIT, GPT, GGT, CRP, WBC, neutrophils, and lymphocytes were examined, while the levels of IL-6, CXCL1, IL-1b, and IL-10 were measured in the saliva.
Thirty liver-transplanted children and 30 healthy children were included in the study. Compared with published data for healthy children, the liver-transplanted group showed similar hepatic serum levels, yet reduced levels of serum inflammatory markers. Compared with the control group, in the transplanted group, the mean value of IL-6 was lower and the mean value of CXCL1 was similar. Interestingly, the anti-inflammatory IL-10 cytokine was lower in the transplanted group, while the pro-inflammatory IL-1β cytokine was higher.
The salivary inflammatory markers examined showed a similar pattern to the serum inflammatory values, though different markers were examined in the serum and saliva.
The current study stresses the potential of oral fluids as an accessible biofluid, for use as a diagnostic substrate for systemic and oral diseases.
0136-16-RMC, Registered on 01 March 2018.
本研究旨在比较肝移植儿童与健康对照组儿童唾液细胞因子谱，作为血液检查的潜在替代方法，并将常用实验室血液检查值与已发表的血液值相关联。
本研究招募了肝移植儿童和相同性别及年龄分布的健康儿童对照组。在为肝移植儿童进行常规血液检查的同一预约时间收集唾液。还从具有相似年龄和性别分布的健康儿童对照组中收集唾液。从文献中提取正常健康血液值进行比较。使用 ELISA 定量检测唾液中的细胞因子水平。分析比较了肝移植儿童和健康儿童的血清和唾液值。在血清中，检查了白蛋白、GIT、GPT、GGT、CRP、白细胞、中性粒细胞和淋巴细胞的水平，而在唾液中测量了 IL-6、CXCL1、IL-1b 和 IL-10 的水平。
本研究纳入了 30 名肝移植儿童和 30 名健康儿童。与健康儿童的已发表数据相比，肝移植组显示出相似的肝血清水平，但血清炎症标志物水平降低。与对照组相比，在移植组中，IL-6 的平均值较低，而 CXCL1 的平均值相似。有趣的是，抗炎性细胞因子 IL-10 在移植组中较低，而促炎性细胞因子 IL-1β 较高。
所检查的唾液炎症标志物与血清炎症值表现出相似的模式，尽管在血清和唾液中检查了不同的标志物。
目前的研究强调了口腔液作为一种可及生物流体的潜力，可作为系统性和口腔疾病的诊断基质。
0136-16-RMC，于 2018 年 3 月 1 日注册。
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