Wang Anyi, Leible Malte, Lin Jun, Weiss Jochen, Zhong Qixin
Department of Food Science, University of Tennessee, Knoxville, Tennessee 37996, United States.
Department of Food Science and Biotechnology, University of Hohenheim, Stuttgart 70174, Germany.
J Agric Food Chem. 2020 Dec 16;68(50):14978-14987. doi: 10.1021/acs.jafc.0c05143. Epub 2020 Nov 3.
Caffeic acid phenethyl ester (CAPE) has various biological activities but low water solubility and poor bioavailability. In this study, CAPE was encapsulated in skim milk powder (SMP) by spray drying warm aqueous ethanol solutions with different mass ratios of SMP and CAPE. The loading capacity and encapsulation efficiency were up to 10.1 and 41.7%, respectively. Differential scanning calorimetry and X-ray diffraction results confirmed the loss of crystallinity of CAPE after encapsulation. Fourier-transform infrared and fluorescence spectroscopy results indicated the hydrophobic binding between CAPE and caseins. Scanning electron microscopy and static light scattering results showed spherical capsules with an average diameter of around 26 μm. The CAPE loaded in SMP microcapsules showed significantly improved bioaccessibility and antiproliferation activity against human colon cancer cells compared to free CAPE. The simple, scalable, and low-cost approach in the present study may be significant for industrial encapsulation of CAPE and other lipophilic bioactive compounds.
咖啡酸苯乙酯（CAPE）具有多种生物活性，但水溶性低且生物利用度差。在本研究中，通过喷雾干燥具有不同质量比的脱脂奶粉（SMP）和CAPE的温热乙醇水溶液，将CAPE包封在脱脂奶粉中。载药量和包封率分别高达10.1%和41.7%。差示扫描量热法和X射线衍射结果证实了CAPE包封后结晶度的丧失。傅里叶变换红外光谱和荧光光谱结果表明CAPE与酪蛋白之间存在疏水结合。扫描电子显微镜和静态光散射结果显示平均直径约为26μm的球形胶囊。与游离CAPE相比，负载在SMP微胶囊中的CAPE对人结肠癌细胞的生物可及性和抗增殖活性显著提高。本研究中简单、可扩展且低成本的方法可能对CAPE和其他亲脂性生物活性化合物的工业包封具有重要意义。
