Hong Shan, Dia Vermont P, Baek Seung Joon, Zhong Qixin
Department of Food Science, The University of Tennessee, Knoxville, TN, USA.
College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
Lebensm Wiss Technol. 2022 Jan 15;154. doi: 10.1016/j.lwt.2021.112751. Epub 2021 Nov 2.
Incorporating lipophilic phytochemicals with anti-cancer activities in functional beverages requires an appropriate nanoencapsulation technology. The present objective was to encapsulate apigenin with whey protein isolate (WPI) utilizing a pH-cycle method and subsequently characterize physicochemical properties, the anticancer activities against human colorectal HCT-116 and HT-29 cancer cells, and the bioavailability. Up to 2.0 mg/mL of apigenin was nanoencapsulated with 1.0 mg/mL WPI, with an encapsulation efficiency of up to 98.15% and loading capacity of up to 196.21 mg/g-WPI. Nanodispersions were stable during storage, and apigenin became amorphous after encapsulation. Nanoencapsulation and digestion did not reduce the anti-proliferative activity of apigenin. Nanoencapsulation of apigenin enhanced the cellular uptake, the pro-apoptotic effects, and the bioavailability in the mice's blood and colon mucosa when comparing to the unencapsulated apigenin. Therefore, the present work may be significant to incorporate lipophilic phytochemicals in functional beverages for disease prevention.
将具有抗癌活性的亲脂性植物化学物质添加到功能性饮料中需要合适的纳米封装技术。当前的目标是利用pH循环法用乳清蛋白分离物（WPI）封装芹菜素，随后表征其物理化学性质、对人结肠HCT - 116和HT - 29癌细胞的抗癌活性以及生物利用度。用1.0 mg/mL的WPI纳米封装了高达2.0 mg/mL的芹菜素，封装效率高达98.15%，负载量高达196.21 mg/g - WPI。纳米分散体在储存期间稳定，封装后芹菜素变为无定形。纳米封装和消化并未降低芹菜素的抗增殖活性。与未封装的芹菜素相比，芹菜素的纳米封装增强了细胞摄取、促凋亡作用以及在小鼠血液和结肠黏膜中的生物利用度。因此，目前的工作对于将亲脂性植物化学物质纳入功能性饮料以预防疾病可能具有重要意义。
