Annušová Adriana, Bodík Michal, Hagara Jakub, Kotlár Mário, Halahovets Yuriy, Mičušík Matej, Chlpík Juraj, Cirák Július, Hofbauerová Monika, Jergel Matej, Majková Eva, Šiffalovič Peter
Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. Centre for Advanced Material Application, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia.
Nanotechnology. 2020 Nov 3;32(4):045708. doi: 10.1088/1361-6528/abc035.
Gel filtration is a versatile technique employed for biological molecules and nanoparticles, offering their reproducible classification based on size and shape. Colloidal nanoparticles are of significant interest in biomedical applications due to a large number of solution-based bioconjugation procedures. Nevertheless, the inherent polydispersity of the nanoparticles produced by various techniques necessitates the employment of high yield separation and purification techniques. Here we demonstrate the employment of gel filtration on non-stoichiometric plasmonic MoO nanoparticles, prepared by an oxidation process during liquid-phase exfoliation of few-layer MoS nanosheets. This resulted in the separation of two types of MoO particles, in the form of two different chromatographic fractions. They showed different sizes, morphological and optical properties. The fraction containing smaller particles with diameters of 1-4 nm, exhibited an increased absorbance peak in the near IR region and responded with a significant temperature increase to laser irradiation at the wavelength close to the maximal absorption. The fraction with the larger particles from 3 up to 10 nm, showed weak photoluminescence and a preferred orientation upon the deposition on a planar substrate. However, it had no absorbance in the near IR compared to the former fraction. According to our knowledge, this is the first time that the gel filtration was applied to the separation of molybdenum oxide nanomaterials. This step ensured the isolation of plasmonic MoO nanoparticles suitable for further bioconjugation and target photothermal treatment.
凝胶过滤是一种用于生物分子和纳米颗粒的通用技术，可根据大小和形状对它们进行可重复的分类。由于大量基于溶液的生物共轭程序，胶体纳米颗粒在生物医学应用中具有重大意义。然而，通过各种技术生产的纳米颗粒固有的多分散性使得必须采用高产率的分离和纯化技术。在此，我们展示了凝胶过滤在非化学计量比的等离子体MoO纳米颗粒上的应用，这些颗粒是在少层MoS纳米片的液相剥离过程中的氧化过程中制备的。这导致了两种类型的MoO颗粒的分离，呈现为两个不同的色谱馏分。它们表现出不同的尺寸、形态和光学性质。含有直径为1 - 4 nm的较小颗粒的馏分在近红外区域表现出吸收峰增加，并且在接近最大吸收波长的激光照射下温度显著升高。含有3至10 nm较大颗粒的馏分在沉积在平面基板上时表现出弱的光致发光和择优取向。然而，与前一个馏分相比，它在近红外区域没有吸收。据我们所知，这是首次将凝胶过滤应用于氧化钼纳米材料的分离。这一步确保了适合进一步生物共轭和靶向光热治疗的等离子体MoO纳米颗粒的分离。
