Tóth Brigitta, Juhász Csaba, Labuschagne Maryke, Moloi Makoena Joyce
Institute of Food Science, University of Debrecen, 138 Böszörményi St., 4032 Debrecen, Hungary.
Arid Land Research Centre, University of Debrecen, 138 Böszörményi St., 4032 Debrecen, Hungary.
Plants (Basel). 2020 Oct 31;9(11):1472. doi: 10.3390/plants9111472.
The recent study was conducted to examine the influence of acidic soil on the activities of ascorbate (APX) and guaiacol peroxidase (POD), proline, protein as well as malon-dialdehyde (MDA) content, in two commercial spring wheat cultivars (PAN3497 and SST806) at different growth stages (tillering and grain filling). A cultivar effect was significant only for MDA content, while the treatment effect was highly significant for proline, protein, and MDA. The sampling time effect was significant for most characteristics. MDA, antioxidative capacity, as well as protein content increased with maturity. At grain filling, MDA and proline contents were significantly higher at pH 5 than pH 6 and 7 for both cultivars, with the highest content in SST806. Similarly, SST806 had significantly higher APX and POD when growing at pH 5. There were no significant differences in protein content at grain filling between either genotype or treatments affected by low pH. This study showed that growth stage and soil pH influence the rate of lipid peroxidation as well as the antioxidative capacity of wheat, with a larger effect at grain filling, at pH 5. Although SST806 had higher proline, POD, and APX content than PAN3497 at this growth stage, this coincided with a very high MDA content. This shows that the high antioxidative capacity observed here, was not associated with a reduction of lipid peroxidation under low soil pH. Further research should, therefore, be done to establish the role of the induced antioxidant system in association with growth and yield in wheat.
最近开展了一项研究，以考察酸性土壤对两个商用春小麦品种（PAN3497和SST806）在不同生长阶段（分蘖期和灌浆期）的抗坏血酸过氧化物酶（APX）和愈创木酚过氧化物酶（POD）活性、脯氨酸、蛋白质以及丙二醛（MDA）含量的影响。品种效应仅对MDA含量有显著影响，而处理效应对脯氨酸、蛋白质和MDA有极显著影响。采样时间效应对大多数特征有显著影响。MDA、抗氧化能力以及蛋白质含量随成熟度增加。在灌浆期，两个品种在pH 5条件下的MDA和脯氨酸含量均显著高于pH 6和pH 7条件下的，且SST806中的含量最高。同样，SST806在pH 5条件下生长时APX和POD显著更高。在灌浆期，低pH影响的基因型或处理之间的蛋白质含量没有显著差异。本研究表明，生长阶段和土壤pH影响小麦的脂质过氧化速率以及抗氧化能力，在灌浆期pH 5条件下影响更大。尽管在该生长阶段SST806的脯氨酸、POD和APX含量高于PAN3497，但这与非常高的MDA含量同时出现。这表明此处观察到的高抗氧化能力与低土壤pH条件下脂质过氧化的降低无关。因此，应进一步开展研究以确定诱导抗氧化系统在小麦生长和产量方面的作用。
