Department of Obstetrics and Gynecology 2, Kawasaki Medical School, Okayama, Japan
AntiCancer Japan Inc, Narita, Japan.
In Vivo. 2020 Nov-Dec;34(6):3241-3245. doi: 10.21873/invivo.12160.
BACKGROUND/AIM: The discovery of the nude mouse model enabled the experimental growth of human-patient tumors. However, the low establishment rate of tumors in nude and other immunodeficient strains of mice has limited wide-spread clinical use.
In order to increase the establishment rate of surgical specimens of patient tumors, we transplanted tumors to nude mice subcutaneously along with large amounts of surrounding tissue of the tumor.
The new transplantation method increased the establishment rate in nude mice to 66% compared to the old method of implanting the surgical tumor specimen with surrounding tissue removed (14%). High stage and presence of metastasis in the patient donor are positively correlated to tumor engraftment in nude mice.
The new method can potentially allow most cancer patients who undergo surgery or biopsy to have their own mouse model for drug-sensitivity testing.
背景/目的：裸鼠模型的发现使患者肿瘤的实验生长成为可能。然而，肿瘤在裸鼠和其他免疫缺陷品系小鼠中的低建立率限制了其广泛的临床应用。
为了提高患者肿瘤手术标本的建立率，我们将肿瘤与大量肿瘤周围组织一起皮下移植到裸鼠中。
与传统的仅植入去除周围组织的手术肿瘤标本的方法（14%）相比，新的移植方法使裸鼠中的建立率提高到了 66%。患者供体的高分期和转移存在与裸鼠中的肿瘤植入呈正相关。
新方法可能使大多数接受手术或活检的癌症患者都能够拥有自己的用于药敏测试的小鼠模型。
