AntiCancer Inc, San Diego, CA, U.S.A.
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, U.S.A.
In Vivo. 2021 Jul-Aug;35(4):1979-1983. doi: 10.21873/invivo.12466.
BACKGROUND/AIM: Sarcomas of the breast are extremely rare malignant tumors and comprise only 5% of all sarcomas and fewer than 1% of breast cancers. Primary osteosarcoma of the breast is histologically indistinguishable from osteosarcoma of the bone. Effective therapies of this recalcitrant disease have not yet been developed.
A patient-derived xenograft (PDX) mouse model of primary osteosarcoma of the breast was established by subcutaneous implantation of the surgical specimen, along with surrounding normal tissue. Hematoxylin and eosin (H&E) staining was performed on paraffin-embedded histological sections of the original tumor resected from the patient and from implanted tumors that grew in nude mice.
Tumors grew in 46 of 51 mice implanted with the original surgical specimen. The H&E-stained slides of the mouse-grown tumor and the original patient tumor matched, both showing large areas of spindle-shaped cells, characteristic of osteosarcoma.
The first PDX mouse model of primary breast osteosarcoma was established which will enable testing of novel therapeutics as well as basic research of osteosarcoma of the breast.
背景/目的：乳腺肉瘤是极为罕见的恶性肿瘤，仅占所有肉瘤的 5%，不到乳腺癌的 1%。乳腺原发性骨肉瘤在组织学上与骨骨肉瘤无法区分。这种难治性疾病尚未开发出有效的治疗方法。
通过将手术标本及其周围正常组织皮下植入，建立了乳腺原发性骨肉瘤患者来源的异种移植（PDX）小鼠模型。对取自患者的原始肿瘤和在裸鼠中生长的植入肿瘤的石蜡包埋组织学切片进行苏木精和伊红（H&E）染色。
将原始手术标本植入的 51 只小鼠中有 46 只生长出肿瘤。小鼠生长的肿瘤和原始患者肿瘤的 H&E 染色切片相匹配，均显示出大片梭形细胞，这是骨肉瘤的特征。
成功建立了首个乳腺原发性骨肉瘤 PDX 小鼠模型，这将使新型治疗方法的测试以及乳腺骨肉瘤的基础研究成为可能。
