College of Animal Science and Technology, Hunan Agricultural University, Changsha Hunan 410128, China.
Hunan Collaborative Innovation Center of Animal Production Safety, Changsha Hunan 410128, China.
Biomed Res Int. 2020 Oct 20;2020:9494528. doi: 10.1155/2020/9494528. eCollection 2020.
The antioxidant function and metabolic profiles in mice after dietary supplementation with methionine were investigated. The results showed that methionine supplementation enhanced liver GSH-Px activity and upregulated Gpx1 expression in the liver and SOD1 and Gpx4 expressions in the jejunum. Nrf2/Keap1 is involved in oxidative stress, and the western blotting data exhibited that dietary methionine markedly increased Keap1 abundance, while failed to influence the Nrf2 signal. Metabolomics investigation showed that methionine administration increased 2-hydroxypyridine, salicin, and asparagine and reduced D-Talose, maltose, aminoisobutyric acid, and inosine 5'-monophosphate in the liver, which are widely reported to involve in oxidative stress, lipid metabolism, and nucleotides generation. In conclusion, our study provides insights into antioxidant function and liver metabolic profiles in response to dietary supplementation with methionine.
研究了膳食补充蛋氨酸后对小鼠的抗氧化功能和代谢谱的影响。结果表明，蛋氨酸补充增强了肝脏 GSH-Px 活性，并上调了肝脏 Gpx1 表达和空肠 SOD1 和 Gpx4 表达。Nrf2/Keap1 参与氧化应激，Western blot 数据表明膳食蛋氨酸显著增加了 Keap1 的丰度，而对 Nrf2 信号没有影响。代谢组学研究表明，蛋氨酸给药增加了肝脏中的 2-羟基吡啶、水杨苷和天冬酰胺，减少了 D-塔罗糖、麦芽糖、氨基异丁酸和肌苷 5'-单磷酸，这些物质广泛报道与氧化应激、脂质代谢和核苷酸生成有关。总之，本研究深入了解了膳食补充蛋氨酸对抗氧化功能和肝脏代谢谱的影响。
