使用可透析荧光标记物在聚丙烯酰胺凝胶上对酶进行定位和纯化。
Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers.
作者信息
Bodhe A M, Vartak H G, Rele M V, Dhamankar V S
机构信息
Division of Biochemical Sciences, National Chemical Laboratory, Poona, India.
出版信息
Anal Biochem. 1987 Jul;164(1):39-43. doi: 10.1016/0003-2697(87)90364-2.
PMID:3314580
Abstract
A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.
摘要
描述了一种通过聚丙烯酰胺凝胶电泳使用可透析的低分子量荧光肽标记物来定位蛋白质/酶的方法。用荧光胺处理酪蛋白的胃蛋白酶消化物制备的标记物在凝胶电泳上显示出几条带，这有助于定位蛋白质。随后可以通过提取纯化定位到的所需蛋白质。
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