CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
ACS Comb Sci. 2020 Dec 14;22(12):867-872. doi: 10.1021/acscombsci.0c00165. Epub 2020 Nov 4.
Nucleic acid aptamers are single-stranded oligonucleotides that may be evolved for affinity and specificity for their targets and can be easily produced, regenerated, and stabilized. In this study, we adapted Ni-NTA (nickle-charged nitrilotriacetic acid) affinity-chromatography in the development of single-stranded DNA aptamers against N-cadherin protein by systematic evolution of ligands by exponential enrichment (SELEX). After ten rounds of selection, two aptamers, designated NS13 and NC23, were selected, which showed low dissociation constants of 93 and 174 nM, respectively. The 5'-carboxyfluorescein-labeled NS13 was used for the sensitive detection of N-cadherin protein by the enzyme-linked oligonucleotide assay (ELONA) method.
核酸适体是单链寡核苷酸，可通过指数富集配体系统进化技术（SELEX）进化出对其靶标的亲和力和特异性，并且易于生产、再生和稳定。在这项研究中，我们通过 Ni-NTA（镍电荷的亚氨基三乙酸）亲和层析，开发了针对 N-钙黏蛋白蛋白的单链 DNA 适体。经过十轮筛选，选择了两个适体，分别命名为 NS13 和 NC23，它们的解离常数分别为 93 和 174 nM。5'-羧基荧光素标记的 NS13 被用于通过酶联寡核苷酸检测（ELONA）方法灵敏检测 N-钙黏蛋白蛋白。
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