College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
National Research Center of Engineering Technology for Utilization of Functional Ingredients From Botanicals, Collaborative Innovation Centre of Utilisation of Functional Ingredients From Botanicals, Hunan Agricultural University, Changsha, 410128, Hunan, China.
Bioprocess Biosyst Eng. 2021 Mar;44(3):429-442. doi: 10.1007/s00449-020-02463-w. Epub 2020 Nov 4.
Exogenous enzymes are extraneous enzymes that are not intrinsic to the subject. The exogenous enzyme industry has been rapidly developing recently. Successful application of recombinant DNA amplification, high-efficiency expression, and immobilization technology to genetically engineered bacteria provides a rich source of enzymes. Amylase, cellulase, protease, pectinase, glycosidase, tannase, and polyphenol oxidase are among the most widely used such enzymes. Currently, the application of exogenous enzyme technology in the development of natural plant resources mainly focuses on improving the taste and flavor of the product, enriching the active ingredient contents, deriving and transforming the structure of a chosen compound, and enhancing the biological activity and utilization of the functional ingredient. In this review, we discuss the application status of exogenous enzyme technology for the development of natural plant resources using typical natural active ingredients from plant, such as resveratrol, steviosides, catechins, mogrosides, and ginsenosides, as examples, to provide basis for further exploitation and utilization of exogenous enzyme technology.
外源性酶是指不属于主体的外源酶。最近，外源性酶工业发展迅速。重组 DNA 扩增、高效表达和固定化技术在基因工程菌中的成功应用，为酶提供了丰富的来源。其中最广泛使用的酶有淀粉酶、纤维素酶、蛋白酶、果胶酶、糖苷酶、单宁酶和多酚氧化酶。目前，外源性酶技术在天然植物资源开发中的应用主要集中在改善产品的口感和风味、丰富活性成分含量、衍生和转化选择化合物的结构以及增强功能成分的生物活性和利用。在本文中，我们讨论了外源酶技术在天然植物资源开发中的应用现状，以植物中的典型天然活性成分如白藜芦醇、甜菊苷、儿茶素、罗汉果苷和人参皂苷为例，为进一步开发和利用外源酶技术提供了依据。
