Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran (IRI).
Int Braz J Urol. 2021 Mar-Apr;47(2):322-332. doi: 10.1590/S1677-5538.IBJU.2020.0171.
We assessed the efficacy and safety of a single injection of three bulking agents over the short- and long-term follow-ups in rabbits. Dermal and preputial matrices were compared with Deflux (DxHA) injection.
Twenty-four rabbits were divided into three groups. Group I (n=8) underwent the injection of a lyophilized dermal matrix (LDM) beneath the seromuscular layer of the bladder wall. Rabbits in group II (n=8) were injected with lyophilized preputial matrix (LPM). Rabbits of group III (n=8) were injected with DxHA as the control group. They were followed up for 1 and 6 months after the injection. Subcutaneous injection of all bulking agents was also performed in nude mice. Biopsies were stained with LCA (leukocyte common antibody), CD68, CD31, and CD34. Scanning electron microscopy (SEM) and MTT assay were also performed.
Immunohistochemistry staining with CD68 and LCA revealed higher inflammation grade in LDM as compared with LPM and DxHA. Fibrosis grade was also higher in LDM both in short- and long-term follow-ups. However, no significant difference was detected in CD31 and CD34 staining between control and experimental groups. SEM analysis showed that the particle size of LPM was more similar to DxHA. MTT assay revealed that cell proliferation was similar in DxHA, LDM, and LPM. In-vivo assay in nude mice model showed more promising results in LPM as compared with LDM.
The long-term results demonstrated that LPM was more similar to Deflux with the least local tissue reaction, inflammation, and fibrosis grade.
我们评估了在短期和长期随访中，一次性注射三种填充剂在兔子中的疗效和安全性。将真皮和包皮基质与 Deflux（DxHA）注射进行比较。
将 24 只兔子分为三组。第 I 组（n=8）在膀胱壁的浆膜肌层下注射冻干真皮基质（LDM）。第 II 组（n=8）的兔子注射冻干包皮基质（LPM）。第 III 组（n=8）的兔子作为对照组注射 DxHA。注射后 1 个月和 6 个月进行随访。所有填充剂均在裸鼠皮下注射。用 LCA（白细胞共同抗体）、CD68、CD31 和 CD34 染色进行活检。还进行了扫描电子显微镜（SEM）和 MTT 检测。
CD68 和 LCA 的免疫组织化学染色显示，与 LPM 和 DxHA 相比，LDM 的炎症程度更高。在短期和长期随访中，LDM 的纤维化程度也更高。然而，在 CD31 和 CD34 染色方面，对照组和实验组之间没有发现显著差异。SEM 分析显示，LPM 的粒径更接近 DxHA。MTT 检测显示，DxHA、LDM 和 LPM 中的细胞增殖相似。在裸鼠模型的体内试验中，LPM 的结果优于 LDM。
长期结果表明，LPM 与 Deflux 更相似，局部组织反应、炎症和纤维化程度最低。
