Nursing homes might create an environment favorable for the transmission of because of the presence of hospitalized elderly, overcrowding and close contacts among people. We aimed at identifying risk factors for colonization and determining the genetic relatedness of isolates demonstrating transmission among people. We investigated 736 swab samples from 92 residents and personnel for the presence of . Swabs from anterior nares and throat were collected quarterly (2018) in a nursing home located in Poland. Genotyping was conducted using the multi-locus variable number of tandem repeats fingerprinting (MLVF) method. We observed high seasonal variation in the proportion of participants colonized with methicillin-resistant Staphylococcus aureus (MRSA) strains (0% to 13.5%). A multivariate analysis revealed that residents aged more than 85 years old are at risk for becoming intermittent carriers ( = 0.013). The MLVF analysis revealed a high genetic diversity among methicillin-sensitive (MSSA) strains and close genetic relatedness between MRSA strains. We proved the advanced aged were predisposed to intermittent carriage. Genotyping revealed the transmission of among the participants living in a closed environment. A high genetic relatedness among isolated MRSA suggests its clonal spread in the nursing home.