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Synthesis of 4R- and 4S-tritium labeled NADPH for the determination of the coenzyme stereospecificity of NADPH: protochlorophyllide oxidoreductase.

作者信息

Valera V, Fung M, Wessler A N, Richards W R

机构信息

Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

出版信息

Biochem Biophys Res Commun. 1987 Oct 14;148(1):515-20. doi: 10.1016/0006-291x(87)91141-7.

DOI:10.1016/0006-291x(87)91141-7
PMID:3314870
Abstract

A rapid and easy method for the production of both the 4R and 4S tritium labeled isomers of either NADH or NADPH has been developed. The method requires the use of only a single labeled compound (D-[1(-3)H] glucose), and two enzymes (glucose dehydrogenase from Bacillus sp. and alcohol dehydrogenase from Thermoanaerobium brockii) which are specific for the pro S and pro R hydrogens, respectively, of either NADH or NADPH. The 4R and 4S tritium labeled isomers of NADPH have been used to determine that NADPH:protochlorophyllide oxidoreductase from etiolated wheat was specific for the pro S hydrogen of NADPH.

摘要

相似文献

1
Synthesis of 4R- and 4S-tritium labeled NADPH for the determination of the coenzyme stereospecificity of NADPH: protochlorophyllide oxidoreductase.
Biochem Biophys Res Commun. 1987 Oct 14;148(1):515-20. doi: 10.1016/0006-291x(87)91141-7.
2
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The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Isolation and characterization of the NADPH:protochlorophyllide oxidoreductase.大麦（Hordeum vulgare L.）的原叶绿素酸酯全色素。NADPH：原叶绿素酸酯氧化还原酶的分离与特性鉴定。
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6
Light modulation of the activity of protochlorophyllide reductase.原叶绿素酸酯还原酶活性的光调节
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7
The light intensity dependence of protochlorophyllide photoconversion and its significance to the catalytic mechanism of protochlorophyllide reductase.原叶绿素酸酯光转化的光强依赖性及其对原叶绿素酸酯还原酶催化机制的意义。
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9
Biochemical characterization and localization of the 36,000-dalton NADPH: protochlorophyllide oxidoreductase in squash etioplasts.西葫芦黄化质体中36000道尔顿的NADPH：原叶绿素酸酯氧化还原酶的生化特性及定位
Prog Clin Biol Res. 1982;102 Pt B:13-23.
10
The protochlorophyllide holochrome of barley (Hordeum vulgare L.). The effect of light on the NADPH:protochlorophyllide oxidoreductase.大麦（Hordeum vulgare L.）的原叶绿素酸酯全色素。光对NADPH：原叶绿素酸酯氧化还原酶的影响。
Eur J Biochem. 1981 Nov;120(1):95-103. doi: 10.1111/j.1432-1033.1981.tb05674.x.

引用本文的文献

1
Stepwise Hydride Transfer in a Biological System: Insights into the Reaction Mechanism of the Light-Dependent Protochlorophyllide Oxidoreductase.逐步氢转移在生物体系中的研究：对依赖于光的原叶绿素氧化还原酶反应机制的深入了解。
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2
Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase.低温和激光光激发研究确定了光驱动酶原叶绿素酸氧化还原酶中活性位点残基的多种作用。
J Biol Chem. 2009 Jul 3;284(27):18160-6. doi: 10.1074/jbc.M109.020719. Epub 2009 May 13.
3
Spectroscopic and kinetic characterization of the light-dependent enzyme protochlorophyllide oxidoreductase (POR) using monovinyl and divinyl substrates.
使用单乙烯基和二乙烯基底物对光依赖性酶原叶绿素酸氧化还原酶（POR）进行光谱和动力学表征。
Biochem J. 2006 Feb 15;394(Pt 1):243-8. doi: 10.1042/BJ20051635.
4
The protochlorophyllide-chlorophyllide cycle.原叶绿素酸酯-叶绿素酸酯循环。
Photosynth Res. 2001;70(3):257-71. doi: 10.1023/A:1014769707404.
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Enzymology below 200 K: the kinetics and thermodynamics of the photochemistry catalyzed by protochlorophyllide oxidoreductase.200K以下的酶学：原叶绿素酸酯氧化还原酶催化的光化学动力学与热力学
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11145-50. doi: 10.1073/pnas.182274199. Epub 2002 Aug 12.
6
The role of protein surface charge in catalytic activity and chloroplast membrane association of the pea NADPH: protochlorophyllide oxidoreductase (POR) as revealed by alanine scanning mutagenesis.丙氨酸扫描诱变揭示豌豆NADPH：原叶绿素酸酯氧化还原酶（POR）的蛋白质表面电荷在催化活性和叶绿体膜结合中的作用。
Plant Mol Biol. 1999 Jan;39(2):309-23. doi: 10.1023/a:1006135100760.