Grachev M A, Lukhtanov E A, Mustaev A A, Abdukaiumov M N, Rabinov I V
Bioorg Khim. 1987 Jul;13(7):992-5.
E. coli RNA polymerase was selectively labelled in the presence of promoters at a histidine residue of the beta-subunit by treatment with GDP beta-imidazolide and then with [alpha-32P]UTP (or [alpha-33P]UTP). Partial cyanogen bromide cleavage of the labelled polypeptide afforded a series of "single-hit" labelled peptides, the electrophoretic pattern of which suggested that the labelling site was His1237. This conclusion was confirmed by a similar pattern obtained with products of the cyanogen bromide cleavage of a radioactive peptide obtained by the limited trypsinolysis (C-terminal peptide consisting of 423 amino acid residues). Interpretation of our earlier results in favour of His1116 as the labelling point (Dokl. Acad. nauk SSSR, 1985, v. 281, p. 723) was incorrect due to the electrophoretic "compression" of three labelled peptide bands.
在启动子存在的情况下，通过用GDP-β-咪唑化物处理，然后用[α-32P]UTP（或[α-33P]UTP）处理，大肠杆菌RNA聚合酶在β亚基的一个组氨酸残基处被选择性标记。对标记多肽进行部分溴化氰裂解，得到一系列“单次打击”标记肽，其电泳图谱表明标记位点是His1237。通过对有限胰蛋白酶消化得到的放射性肽（由423个氨基酸残基组成的C末端肽）进行溴化氰裂解产物获得的类似图谱证实了这一结论。我们早期支持His1116作为标记点的结果（苏联科学院报告，1985年，第281卷，第723页）的解释是不正确的，因为三个标记肽带的电泳“压缩”。
