• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用台式离心机生产的富血小板血浆的质量评估及其与标准化市售富血小板血浆的比较。

Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma.

作者信息

Srinivasan V Ramesh, Rekha M, Edsor Effie, Raja S Pradheep, Kumar T Dinesh, Kalaiselvan S

机构信息

Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India.

Department of Oral and Maxillofacial Pathology, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India.

出版信息

J Pharm Bioallied Sci. 2020 Aug;12(Suppl 1):S607-S612. doi: 10.4103/jpbs.JPBS_163_20. Epub 2020 Aug 28.

DOI:10.4103/jpbs.JPBS_163_20
PMID:33149529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7595503/
Abstract

AIMS

The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP.

MATERIALS AND METHODS

REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM).

STATISTICAL ANALYSIS USED

The test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated.

RESULTS

The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good.

CONCLUSIONS

With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/μL, the clinical results were good with desired bone formation, thereby providing good avenue for further research.

摘要

目的

本研究旨在评估通过内部台式离心机方法制备的富血小板血浆（PRP）的质量，并将其与标准化商业PRP的质量进行比较。

材料与方法

使用雷米台式离心机制备PRP，并通过使用贝克曼库尔特细胞计数器计算10个PRP样本中的血小板数量，以及使用日本电子JEM透射电子显微镜（TEM）评估血小板的形态质量，与标准化商业PRP进行比较。

所用统计分析

台式PRP中血小板计数与治疗性PRP测试值的检验值为12.618。P值<0.001，具有统计学意义。在台式离心机血小板计数的正态Q-Q图中，数据呈正态分布。因此，测试样本偏差不大。

结果

血小板计数低于标准化商业PRP。在日本电子JEM透射电子显微镜下观察，血小板中的α颗粒完整，PRP的形态质量良好。

结论

通过本研究，我们确定通过内部台式离心机方法制备的PRP的形态质量与标准化商业PRP相当。尽管血小板数量少于100万个细胞/μL，但临床结果良好，骨形成理想，从而为进一步研究提供了良好的途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/298137b56dd6/JPBS-12-607-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/d1652f26bbef/JPBS-12-607-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/40e76ce49323/JPBS-12-607-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/4ba8c74c8042/JPBS-12-607-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/36053e1f01ac/JPBS-12-607-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/1ed0cca9db61/JPBS-12-607-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/52b5bffeb02d/JPBS-12-607-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/f7812264e768/JPBS-12-607-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/e801017a9d42/JPBS-12-607-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/0761c9d32279/JPBS-12-607-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/524d2351e96a/JPBS-12-607-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/298137b56dd6/JPBS-12-607-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/d1652f26bbef/JPBS-12-607-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/40e76ce49323/JPBS-12-607-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/4ba8c74c8042/JPBS-12-607-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/36053e1f01ac/JPBS-12-607-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/1ed0cca9db61/JPBS-12-607-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/52b5bffeb02d/JPBS-12-607-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/f7812264e768/JPBS-12-607-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/e801017a9d42/JPBS-12-607-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/0761c9d32279/JPBS-12-607-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/524d2351e96a/JPBS-12-607-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dcb3/7595503/298137b56dd6/JPBS-12-607-g011.jpg

相似文献

1
Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma.使用台式离心机生产的富血小板血浆的质量评估及其与标准化市售富血小板血浆的比较。
J Pharm Bioallied Sci. 2020 Aug;12(Suppl 1):S607-S612. doi: 10.4103/jpbs.JPBS_163_20. Epub 2020 Aug 28.
2
The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane.离心机特性和离心方案对富含白细胞和血小板纤维蛋白（L-PRF）凝块和膜中细胞、生长因子和纤维蛋白结构的影响。
Platelets. 2018 Mar;29(2):171-184. doi: 10.1080/09537104.2017.1293812. Epub 2017 Apr 24.
3
[Preparation of platelet-rich plasma gel and its effect on skin flap survival of rat].富血小板血浆凝胶的制备及其对大鼠皮瓣存活的影响
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2012 Jan;26(1):64-9.
4
A Comparison of Platelet Count and Enrichment Percentages in the Platelet Rich Plasma (PRP) Obtained Following Preparation by Three Different Methods.三种不同方法制备的富血小板血浆（PRP）中血小板计数及富集百分比的比较
J Clin Diagn Res. 2015 Feb;9(2):ZC10-2. doi: 10.7860/JCDR/2015/11011.5536. Epub 2015 Feb 1.
5
Prospective Evaluation of Feline Sourced Platelet-Rich Plasma Using Centrifuge-Based Systems.使用基于离心机的系统对猫源富血小板血浆进行前瞻性评估。
Front Vet Sci. 2020 Jun 12;7:322. doi: 10.3389/fvets.2020.00322. eCollection 2020.
6
[Experimental research on the effects of different activators on the formation of platelet-rich gel and the release of bioactive substances in human platelet-rich plasma].不同激活剂对人富血小板血浆中富血小板凝胶形成及生物活性物质释放影响的实验研究
Zhonghua Shao Shang Za Zhi. 2017 Jan 20;33(1):12-17. doi: 10.3760/cma.j.issn.1009-2587.2017.01.004.
7
A Modified Preparation Method of Ideal Platelet-Rich Fibrin Matrix From Whole Blood.一种从全血制备理想富血小板纤维蛋白基质的改良方法。
Front Med (Lausanne). 2021 Aug 13;8:724488. doi: 10.3389/fmed.2021.724488. eCollection 2021.
8
[Preparation of autologous platelet-rich gel for diabetic refractory dermal ulcer and growth factors analysis from it].[自体富血小板凝胶制备用于糖尿病难愈性皮肤溃疡及其生长因子分析]
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2008 Apr;22(4):468-71.
9
Comparison of point-of-care methods for preparation of platelet concentrate (platelet-rich plasma).比较即时检测方法在制备血小板浓缩物（富血小板血浆）中的应用。
Int J Oral Maxillofac Implants. 2012 Jul-Aug;27(4):762-9.
10
Anti-Inflammatory Effects of Novel Standardized Platelet Rich Plasma Releasates on Knee Osteoarthritic Chondrocytes and Cartilage in vitro.新型标准化富血小板血浆释放物对体外膝骨关节炎软骨细胞和软骨的抗炎作用。
Curr Pharm Biotechnol. 2019;20(11):920-933. doi: 10.2174/1389201020666190619111118.

引用本文的文献

1
Platelet-Rich Plasma Improves Pregnancy Outcomes in Moderate to Severe Intrauterine Adhesion: A Retrospective Cohort Study.富血小板血浆改善中重度宫腔粘连患者的妊娠结局：一项回顾性队列研究
J Clin Med. 2023 Feb 7;12(4):1319. doi: 10.3390/jcm12041319.

本文引用的文献

1
Effects of platelet-rich plasma on the healing of autologous bone grafted mandibular defects in dogs.富血小板血浆对犬自体骨移植下颌骨缺损愈合的影响。
J Oral Maxillofac Surg. 2006 Mar;64(3):443-51. doi: 10.1016/j.joms.2005.11.016.
2
Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: an in vitro study.富血小板血浆（PRP）浓度对牙槽骨细胞活力和增殖的影响：一项体外研究。
Int J Oral Maxillofac Surg. 2005 Jun;34(4):420-4. doi: 10.1016/j.ijom.2004.10.018. Epub 2005 Jan 27.
3
Activation of platelet-rich plasma using thrombin receptor agonist peptide.
使用凝血酶受体激动肽激活富血小板血浆。
J Oral Maxillofac Surg. 2005 Apr;63(4):529-35. doi: 10.1016/j.joms.2004.12.007.
4
Differential growth factor retention by platelet-rich plasma composites.富含血小板血浆复合材料对生长因子的差异性保留
J Oral Maxillofac Surg. 2005 Apr;63(4):521-8. doi: 10.1016/j.joms.2004.09.012.
5
Comparison of the platelet concentrate collection system with the plasma-rich-in-growth-factors kit to produce platelet-rich plasma: a technical report.用于制备富血小板血浆的血小板浓缩物采集系统与富含生长因子血浆试剂盒的比较：技术报告
Int J Oral Maxillofac Implants. 2005 Jan-Feb;20(1):118-23.
6
Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing.富血小板血浆的血小板定量及生长因子分析：对伤口愈合的意义
Plast Reconstr Surg. 2004 Nov;114(6):1502-8. doi: 10.1097/01.prs.0000138251.07040.51.
7
Platelet-rich plasma: evidence to support its use.富血小板血浆：支持其应用的证据。
J Oral Maxillofac Surg. 2004 Apr;62(4):489-96. doi: 10.1016/j.joms.2003.12.003.
8
The Harvest Smart PRePTM system versus the Friadent-Schütze platelet-rich plasma kit.Harvest Smart PRePTM系统与Friadent-Schütze富血小板血浆试剂盒的对比
Clin Oral Implants Res. 2003 Apr;14(2):233-9. doi: 10.1034/j.1600-0501.2003.140215.x.
9
Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration.用于骨再生局部应用的自体浓缩富血小板血浆（cPRP）。
Int J Oral Maxillofac Surg. 2002 Dec;31(6):615-9. doi: 10.1054/ijom.2002.0322.
10
Platelet-rich plasma: harvesting with a single-spin centrifuge.
J Oral Implantol. 2002;28(6):297-301. doi: 10.1563/1548-1336(2002)028<0297:PPHWAS>2.3.CO;2.