Antibiotic resistance and typing of locus in isolated from clinical samples in Sanandaj, Western Iran.

OBJECTIVES

Infections by remain an important health problem. The aims were to detect , staphylococcal cassette chromosome mec (SCCmec), , and integrons in and to investigate the relationship of types with antibiotic resistance of isolates.

MATERIALS AND METHODS

In this cross-sectional study, 70 isolates were collected between December 2017 and May 2018 from clinical specimens of patients in two hospitals of Sanandaj, western Iran. Susceptibility was determined by disk diffusion for 9 antibiotics and by vancomycin E test. The , classes 1-3 integrons, SCC I-V, and I-IV were detected by polymerase chain reaction. A -value<0.05 was considered significant.

RESULTS

The most effective antibiotics were linezolid, vancomycin, and trimethoprim-sulfamethoxazole (above 90% sensitivity). Of the 70 isolates, 17.1% were methicillin-resistant (MRSA), 8.6% carried class 1 integron, 11.4% carried , 17.1% carried I, and 30% carried III. SCC III and SCCV were detected. An association was found between resistance to certain antibiotics and the presence of I (-value<0.05). Conversely, the prevalence of III in susceptible strains was higher than non-susceptible strains, and no MRSA isolates belonged to III (-value<0.05).

CONCLUSION

These data suggest that activity may influence the resistance of to antibiotics. Although the prevalence of and integron was relatively low, the identification of such strains calls for serious health concerns; thus highlights the need to monitor drug resistance in .