二十二碳六烯酸（DHA）在富含雨生红球藻生物量的干燥犬粮中的分析：AOAC 方法 996.06 的基质扩展验证和确认。
The Analysis of Docosahexaenoic Acid (DHA) in Dried Dog Food Enriched with an Aurantiochytrium limacinum Biomass: Matrix Extension Validation and Verification of AOAC Method 996.06.
机构信息
Regulatory Affairs Department, Alltech Ireland, Summerhill Rd., Dunboyne, County Meath, Ireland.
Research Department, Alltech Inc., Nicholasville, KY, USA.
出版信息
J AOAC Int. 2021 Mar 5;104(1):68-77. doi: 10.1093/jaoacint/qsaa097.
BACKGROUND
Docosahexaenoic acid (DHA) plays an important role in brain and retinal development in dogs. However, supranutritional dietary supplementation can result in health issues, including gastrointestinal bleeding, making the accurate analysis of DHA in dog food important for nutritional and welfare regulatory compliance.
OBJECTIVE
The aim of this study was to conduct a validation and verification of the AOAC 996.06 method, and hence establish its fitness for purpose, for the analysis of DHA in dried dog food supplemented with a heterotrophically grown unextracted DHA-rich Aurantiochytrium limacinum biomass.
METHODS
The AOAC 996.06 method, which involves the use of gas chromatography coupled to flame ionization detection (GC-FID), was used to conduct a validation of the analysis of DHA in dried dog food and the results were verified in a second laboratory.
RESULTS
The method was found to be linear over the ranges analyzed and results were found to be within the acceptance criteria for precision and accuracy, verifying the applicability for this matrix. The selectivity and sensitivity of the method were also determined.
CONCLUSIONS
The AOAC 996.06 method is fit for purpose for the analysis of DHA in dry dog food kibble.
HIGHLIGHTS
The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternative manufacturing methods, i.e. pelleted and extruded with no significant matrix effects being observed.
背景
二十二碳六烯酸（DHA）在犬类大脑和视网膜发育中起着重要作用。然而，超营养饮食补充可能会导致健康问题，包括胃肠道出血，因此准确分析犬粮中的 DHA 对于营养和福利法规的合规性非常重要。
目的
本研究旨在对 AOAC 996.06 方法进行验证和确认，从而确定其在分析添加了异养生长的未经提取的富含 DHA 的雨生红球藻生物质的干燥犬粮中的 DHA 时的适用性。
方法
采用气相色谱法结合火焰离子化检测（GC-FID）的 AOAC 996.06 方法，对干燥犬粮中 DHA 的分析进行了验证，并在第二个实验室进行了验证。
结果
该方法在分析范围内呈线性，结果在精密度和准确度的可接受标准内，验证了该方法在该基质中的适用性。该方法的选择性和灵敏度也得到了确定。
结论
AOAC 996.06 方法适用于分析干燥型犬粮中的 DHA。
重点
该方法可应用于各种犬粮样品，补充未经提取的雨生红球藻生物质，采用替代的制造方法，如颗粒状和挤压状，不会观察到明显的基质效应。
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