Bureau J P, Labrecque G, Coupé M, Garrelly L
Laboratoire de cytologie, Faculté de Médecine, Montpellier-Nîmes, France.
Chronobiol Int. 1986;3(1):23-8. doi: 10.3109/07420528609083156.
The temporal variation in the migration of polymorphonuclear leukocytes (PMN) induced by live BCG was studied in the mouse. Ten microliter of a 5 X 10(6) live BCG/ml suspension or sterile saline were placed on a cell trap immediately before its subcutaneous implantation at different clock times: 0100, 0500, 0900, 1300, 1700 and 2100 in animals synchronized with L(0700-1900): D(1900-0700). Eight hours later, the cell trap was removed, prepared for histological identification and counted. PMN counts in the cell trap were maximal 480 min after implantation. Non-specific migration was thought to occur and the peak value of leukocytes of 22.8 +/- 6.1 cells/10,000 micron 2 was obtained when the saline cell-trap was implanted at 0500. In the BCG-treated mice, a circadian rhythm was observed in the migration of leukocytes. The acrophase was at 1700. The results support the hypothesis that the circadian stage of antigen encounter influences the magnitude of the immune response.
在小鼠中研究了活卡介苗诱导的多形核白细胞（PMN）迁移的时间变化。在不同的时钟时间（0100、0500、0900、1300、1700和2100），即在与L（0700 - 1900）：D（1900 - 0700）同步的动物皮下植入细胞阱之前，立即将10微升5×10⁶活卡介苗/毫升悬浮液或无菌盐水置于细胞阱上。8小时后，取出细胞阱，准备进行组织学鉴定并计数。植入后480分钟时细胞阱中的PMN计数最高。当在0500植入盐水细胞阱时，认为发生了非特异性迁移，白细胞峰值为22.8±6.1个细胞/10000平方微米。在卡介苗处理的小鼠中，观察到白细胞迁移存在昼夜节律。高峰期在1700。结果支持抗原接触的昼夜阶段影响免疫反应强度这一假设。
