Liu C, Li X, Song G X, Hua H J, Gong Q X, Wang Z, Fan Q H
Department of Pathology, Jiangsu Province People's Hospital(the First Affiliated Hospital of Nanjing Medical University), Nanjing 210029, China.
Zhonghua Bing Li Xue Za Zhi. 2020 Nov 8;49(11):1158-1162. doi: 10.3760/cma.j.cn112151-20200221-00116.
To study the clinicopathological significance of cyclin D1 expression in Rosai-Dorfman disease (RDD). Seventeen cases of RDD were evaluated by HE, immunohistochemical staining and molecular genetic analysis. Expression of cyclin D1 was compared between RDDs and control group that included 29 cases of reactive histiocytosis, 9 cases of IgG4-related disease, and 2 cases of Erdheim-Chester disease. Cyclin D1 was expressed in RDDs (17/17), reactive histiocytosis (11/29), IgG4-related diseases (3/9), and Erdheim-Chester disease (2/2), respectively, with nuclear staining in the RDD cells or proliferative histiocytes. Chi-square test showed that expression of cyclin D1 was significantly higher in RDDs than in reactive histiocytosis and IgG4-related diseases (<0.01), but not in Erdheim-Chester diseases (>0.05). The expression threshold for recalculating the percentage of cyclin D1 positive cells was 27.5% (AUC=0.981<0.01) by ROC curve. However, CCND1 gene had no rearrangement detected by fluorescence in situ hybridization, but with increased copies of gene in some RDD cells. ARMS-PCR analysis also did not detect KRAS, BRAF and NRAS gene mutations in any cases. Cyclin D1 may serve as an additional diagnostic marker for RDDs. Its high expression may be related to activation of MAPK pathway, but the pathogenetic significance of cyclin D1 in RDDs needs further study.
研究细胞周期蛋白D1在Rosai-Dorfman病（RDD）中表达的临床病理意义。对17例RDD患者进行HE染色、免疫组化染色及分子遗传学分析。比较RDD组与对照组（包括29例反应性组织细胞增多症、9例IgG4相关性疾病和2例Erdheim-Chester病）中细胞周期蛋白D1的表达情况。细胞周期蛋白D1在RDD（17/17）、反应性组织细胞增多症（11/29）、IgG4相关性疾病（3/9）和Erdheim-Chester病（2/2）中均有表达，在RDD细胞或增殖性组织细胞中呈核染色。卡方检验显示，RDD中细胞周期蛋白D1的表达明显高于反应性组织细胞增多症和IgG4相关性疾病（<0.01），但与Erdheim-Chester病相比无明显差异（>0.05）。通过ROC曲线计算，细胞周期蛋白D1阳性细胞百分比的重新计算表达阈值为27.5%（AUC=0.981<0.01）。然而，荧光原位杂交未检测到CCND1基因重排，但部分RDD细胞中基因拷贝数增加。ARMS-PCR分析在所有病例中均未检测到KRAS、BRAF和NRAS基因突变。细胞周期蛋白D1可作为RDD的一个额外诊断标志物。其高表达可能与MAPK通路激活有关，但细胞周期蛋白D1在RDD中的致病意义有待进一步研究。
