采用分散液液微萃取-高效液相色谱荧光检测法同时测定葡萄酒中的褪黑素和反式白藜芦醇。
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid-liquid microextraction followed by HPLC-FLD.
机构信息
LAQV/REQUIMTE, Laboratório de Bromatologia e Hidrologia, Departamento de Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, 4099-313 Porto, Portugal; Faculdade de Ciências da Nutrição e Alimentação da Universidade do Porto, 4200-465 Porto, Portugal.
LAQV/REQUIMTE, Laboratório de Bromatologia e Hidrologia, Departamento de Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, 4099-313 Porto, Portugal.
出版信息
Food Chem. 2021 Mar 1;339:128091. doi: 10.1016/j.foodchem.2020.128091. Epub 2020 Sep 12.
The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 2 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL) and trans-RSV (169-2616 ng mL) in different wine varieties. Comparison with literature point the overall advantages of the new method.
在葡萄酒中发现褪黑素（Mel）后，人们对葡萄酒的健康益处和消费产生了新的兴趣，通常归因于反式白藜芦醇（trans-RSV）。在此背景下，建立了一种用于通过 LC-FLD 分析葡萄酒中 Mel 和 trans-RSV 的分散液-液微萃取方法。采用 2 因素设计确定显著变量（p<0.05），并采用中心复合设计实现最佳条件：300 µL 氯仿（萃取溶剂）、1500 µL 乙腈（分散溶剂）和 1500 mg NaCl（离子强度）。使用空白基质获得了极好的线性（R>0.9999）、重复性（<3.55%）和准确性（<7.18%），使用葡萄酒进行回收率（>91.9%）。该方法成功应用于不同品种葡萄酒中 Mel（0.63-7.44 ng mL）和 trans-RSV（169-2616 ng mL）的分析。与文献的比较突显了新方法的整体优势。
Zotero 插件