用于根面龋修复的载抗菌肽纳米材料的研究，以抑制牙周炎护理中与牙周炎相关的病原体。

Studies on antimicrobial peptide-loaded nanomaterial for root caries restorations to inhibit periodontitis related pathogens in periodontitis care.

机构信息

Department of Stomatology, College of Stomatology, Southern Medical University, Guangzhou, China.

Institute of Molecular Medicine, Medical College of Eastern Liaoning University, Dandong, China.

出版信息

J Microencapsul. 2021 Mar;38(2):89-99. doi: 10.1080/02652048.2020.1842528. Epub 2021 Jan 5.

DOI:10.1080/02652048.2020.1842528

PMID:33153344

Abstract

AIMS

To prepare a novel antimicrobial peptide Nal-P-113 loaded poly (ethylene glycol) combined chitosan nanoparticles (Nal-P-113-PEG-CSNPs) for root caries restorations to control the periodontitis related pathogens in periodontitis care.

METHODS

Nanoparticles were prepared by simple polymerisation method and characterised using effective analytical methods (TEM, UV, etc.). The antimicrobial activity and biofilm formation of Nal-P-113-PEG-CSNPs was tested against periodontal bacterial pathogens by different methods.

RESULTS

The size of Nal-P-113 loaded PEG-Chitosn nanoparticles was 216.2 ± 1.6 nm. The drug encapsulation efficiency (%EE (w/w) of Nal-P-113-PEG-CSNPs was found to be 89.33 ± 1.67% (w/w). The antimicrobial examination showed that prepared NPs have effectively inhibited the growth of , , and with the MIC of 23 µg/mL, 6 µg/mL and 31 µg/mL, respectively.

CONCLUSIONS

The prepared antimicrobial peptide-loaded PEG-CSNPs provide excellent efficiency but, further studies are necessary to confirm its therapeutic efficacy on periodontitis care.

摘要

目的

制备一种新型的载有抗菌肽 Nal-P-113 的聚乙二醇结合壳聚糖纳米粒（Nal-P-113-PEG-CSNPs），用于根面龋修复，以控制牙周病护理中与牙周病相关的病原体。

方法

采用简单的聚合方法制备纳米粒，并采用有效的分析方法（TEM、UV 等）进行表征。通过不同方法测试 Nal-P-113-PEG-CSNPs 对牙周致病菌的抗菌活性和生物膜形成。

结果

载有 Nal-P-113 的 PEG-Chitosn 纳米粒的粒径为 216.2 ± 1.6nm。Nal-P-113-PEG-CSNPs 的药物包封效率（%EE（w/w））为 89.33 ± 1.67%（w/w）。抗菌试验表明，所制备的纳米粒能有效抑制、、和的生长，其 MIC 分别为 23μg/mL、6μg/mL 和 31μg/mL。