Yu Xiaoxiao, He Man, Chen Beibei, Hu Bin
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
Anal Chim Acta. 2020 Nov 15;1137:191-207. doi: 10.1016/j.aca.2020.07.041. Epub 2020 Jul 24.
The content, migration and transformation of trace elements in living organisms play an important role in physiological activities. The analysis of trace elements in cells is of great significance to understand the function mechanism of trace elements in cells and organisms. Compared with conventional bulk analysis, many medical and clinical researches benefit from cell heterogeneity via single-cell analysis. Time-resolved analysis-inductively coupled plasma-mass spectrometry (TRA-ICP-MS) based strategies have the potential for both counting and quantitative assessment. It has been used to evaluate cell-to-cell difference in elemental content, cellular uptake of metal-containing drugs and nanoparticles. This review focuses on TRA-ICP-MS based methodologies for single-cell analysis over the past few decades. Recent advances in ICP-MS instrumentations (cell introduction systems and mass spectrometers), microfluidic platforms and their in-depth applications in single-cell analysis are summarized. This review also prospects future developments of TRA-ICP-MS for single-cell analysis.
微量元素在生物体内的含量、迁移和转化在生理活动中起着重要作用。细胞中微量元素的分析对于理解微量元素在细胞和生物体中的功能机制具有重要意义。与传统的整体分析相比，许多医学和临床研究受益于通过单细胞分析实现的细胞异质性。基于时间分辨分析-电感耦合等离子体质谱（TRA-ICP-MS）的策略具有计数和定量评估的潜力。它已被用于评估细胞间元素含量的差异、含金属药物和纳米颗粒的细胞摄取。本文综述了过去几十年来基于TRA-ICP-MS的单细胞分析方法。总结了ICP-MS仪器（细胞引入系统和质谱仪）、微流控平台的最新进展及其在单细胞分析中的深入应用。本文还展望了TRA-ICP-MS在单细胞分析方面的未来发展。
