慢性子宫内膜炎中子宫内膜辅助性 T 细胞亚群的改变。
Alteration in endometrial helper T-cell subgroups in chronic endometritis.
机构信息
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Shiga, Japan.
Department of Fundamental Biosciences, Shiga University of Medical Science, Shiga, Japan.
出版信息
Am J Reprod Immunol. 2021 Mar;85(3):e13372. doi: 10.1111/aji.13372. Epub 2020 Nov 25.
PROBLEM
The effect of chronic endometritis (CE) on the subpopulation of CD4 T cells, Th1, Th2, Th17, and regulatory T cells in the endometrium is unknown.
METHOD OF STUDY
Lymphocytes were isolated from the endometrium of CE patients (n = 12) and non-CE patients (n = 7). The CD4 T-cell profile was analyzed by flow cytometry and immunofluorescence.
RESULTS
In the endometrium of CE patients, there were significantly more Th1 cells among CD4 cells and fewer Th2 cells in comparison to non-CE patients. No marked difference was observed in Th17 cells or Foxp3 Treg cells. Moreover, the proportion of Th1 cells increased and the proportion of Th2 cells decreased as the number of CD138 cells increased. Furthermore, when the localization of CD138 cells and CD4 cells was examined, CD4 cells were found to be clustered around CD138 cells in CE patients.
CONCLUSION
The CD4 T-cell profile in the endometrium is altered in women with CE. This finding may help to clarify the pathophysiology and development of treatment methods for CE.
问题
慢性子宫内膜炎（CE）对子宫内膜中 CD4 T 细胞亚群、Th1、Th2、Th17 和调节性 T 细胞的影响尚不清楚。
方法
从 CE 患者（n=12）和非 CE 患者（n=7）的子宫内膜中分离淋巴细胞。通过流式细胞术和免疫荧光分析 CD4 T 细胞谱。
结果
与非 CE 患者相比，CE 患者的 CD4 细胞中 Th1 细胞明显更多，而 Th2 细胞明显更少。Th17 细胞或 Foxp3 Treg 细胞无明显差异。此外，随着 CD138 细胞数量的增加，Th1 细胞的比例增加，Th2 细胞的比例减少。此外，当检查 CD138 细胞和 CD4 细胞的定位时，发现在 CE 患者中，CD4 细胞聚集在 CD138 细胞周围。
结论
CE 患者的子宫内膜中 CD4 T 细胞谱发生改变。这一发现可能有助于阐明 CE 的病理生理学和治疗方法的发展。
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