Reproductive Medicine Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Front Immunol. 2021 Oct 4;12:748447. doi: 10.3389/fimmu.2021.748447. eCollection 2021.
To investigate the Interaction between chronic endometritis (CE) caused endometrial microbiota disorder and endometrial immune environment change in recurrent implantation failure (RIF).
Transcriptome sequencing analysis of the endometrial of 112 patients was preform by using High-Throughput Sequencing. The endometrial microbiota of 43 patients was analyzed by using 16s rRNA sequencing technology.
In host endometrium, CD4 T cell and macrophage exhibited significant differences abundance between CE and non-CE patients. The enrichment analysis indicated differentially expressed genes mainly enriched in immune-related functional terms. and were significantly high infiltration in CE patients, and active in pathways related to carbohydrate metabolism and/or fat metabolism. The increased synthesis of lipopolysaccharide, an important immunomodulator, was the result of microbial disorders in the endometrium.
The composition of endometrial microorganisms in CE and non-CE patients were significantly different. and mainly regulated immune cells by interfering with the process of carbohydrate metabolism and/or fat metabolism in the endometrium. CE endometrial microorganisms might regulate Th17 response and the ratio of Th1 to Th17 through lipopolysaccharide (LPS).
探讨慢性子宫内膜炎（CE）引起的子宫内膜微生物群失调与复发性种植失败（RIF）子宫内膜免疫环境变化的相互作用。
采用高通量测序技术对 112 例患者的子宫内膜进行转录组测序分析。采用 16s rRNA 测序技术分析 43 例患者的子宫内膜微生物群。
在宿主子宫内膜中，CD4 T 细胞和巨噬细胞在 CE 和非 CE 患者之间表现出显著的丰度差异。差异表达基因的富集分析主要富集在与免疫相关的功能术语中。CE 患者中 和 的浸润明显较高，在与碳水化合物代谢和/或脂肪代谢相关的途径中活性较高。内毒素脂多糖是一种重要的免疫调节剂，其合成增加是子宫内膜微生物失调的结果。
CE 和非 CE 患者的子宫内膜微生物组成存在显著差异。和主要通过干扰子宫内膜中碳水化合物代谢和/或脂肪代谢过程来调节免疫细胞。CE 子宫内膜微生物可能通过脂多糖（LPS）调节 Th17 反应和 Th1/Th17 比值。
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