Instituto de Investigaciones Agropecuarias, INIA-La Platina, Santa Rosa 11,610, Santiago, Chile.
Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Camino La Pirámide 5750, Huechuraba, Santiago, Chile.
J Econ Entomol. 2021 Feb 9;114(1):90-99. doi: 10.1093/jee/toaa251.
Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.
快速且经济有效地鉴定 Naupactus 物种正成为智利和其他国家出口柑橘类水果的关键环节，因为一些 Naupactus 属的世界性物种受到检疫法规的限制。本研究旨在开发一种针对 Naupactus cervinus（鞘翅目：象甲科）（Boheman）和 Naupactus xanthographus（鞘翅目：象甲科）（Germar）的快速灵敏检测方案，基于多重 TaqMan Real-time 聚合酶链反应。N. cervinus 和 N. xanthographus 的引物和探针组在 1 pg/μl 至 1 × 10-6 pg/μl 的线性范围内均能实现种特异性检测，模板 DNA 的检测下限低至 160 个拷贝。未检测到非靶标扩增，由 480 个测试样本组成的面板与各自的形态鉴定完全一致，符合率为 100%。
