Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Instituto Nacional da Propriedade Industrial, Rio de Janeiro, Brazil.
J Glob Antimicrob Resist. 2020 Dec;23:331-333. doi: 10.1016/j.jgar.2020.10.015. Epub 2020 Nov 3.
The present study reports the draft genome sequence of Staphylococcus aureus 4181, a strain involved in bovine mastitis that produces aureocin 4181, a broad-spectrum antimicrobial peptide (AMP). Inhibition of multidrug-resistant (MDR) staphylococci involved in human infections by S. aureus 4181 was also investigated.
A sequencing library was constructed using a Nextera XT DNA Library Preparation Kit (Illumina). Whole-genome shotgun sequencing was performed using an Illumina MiSeq System. The A5-miseq pipeline was employed for de novo genome assembly. Genome annotation was performed by the RAST server. The online automated tools BAGEL4 and antiSMASH v.5.0 were used for mining gene clusters encoding AMP production. The virulence potential of the strain was investigated employing online tools. Its inhibitory activity toward MDR staphylococcal isolates associated with human infections was tested by the deferred antagonism assay on brain-heart infusion agar medium.
The total scaffold size was determined to be 2 719 949 bp, with a G + C content of 32.7%. Genome analyses revealed 2504 protein-coding sequences and 74 RNA-coding sequences as well as several genes encoding drug resistance and a single AMP gene cluster coding for aureocin 4181. Staphylococcus aureus 4181 exhibited a pathogenic potential and inhibited all MDR staphylococcal isolates tested as a target.
This study describes the main features of the draft genome of S. aureus 4181, a strain that produces the third four-component bacteriocin described in the literature, namely aureocin 4181. This bacteriocin is a potential alternative drug to control MDR staphylococcal isolates involved in human infections.
本研究报告了金黄色葡萄球菌 4181 的基因组草图序列，该菌株与奶牛乳腺炎有关，能产生广谱抗菌肽（AMP） aureocin 4181。还研究了金黄色葡萄球菌 4181 对涉及人类感染的多药耐药（MDR）葡萄球菌的抑制作用。
使用 Nextera XT DNA 文库制备试剂盒（Illumina）构建测序文库。使用 Illumina MiSeq 系统进行全基因组 shotgun 测序。使用 A5-miseq 管道进行从头基因组组装。通过 RAST 服务器进行基因组注释。使用在线自动化工具 BAGEL4 和 antiSMASH v.5.0 挖掘编码 AMP 产生的基因簇。使用在线工具研究该菌株的毒力潜力。通过在脑心浸液琼脂培养基上进行延迟拮抗试验，测试该菌株对与人类感染相关的 MDR 葡萄球菌分离株的抑制活性。
总支架大小确定为 2 719 949 bp，G + C 含量为 32.7%。基因组分析显示，该菌株有 2504 个编码蛋白的序列和 74 个编码 RNA 的序列，以及几个编码耐药性的基因和一个编码 aureocin 4181 的单一 AMP 基因簇。金黄色葡萄球菌 4181 表现出致病潜力，并抑制了所有测试的 MDR 葡萄球菌分离株作为靶标。
本研究描述了金黄色葡萄球菌 4181 基因组草图的主要特征，该菌株产生了文献中描述的第三种四组分细菌素 aureocin 4181。这种细菌素可能是一种替代药物，可以控制涉及人类感染的 MDR 葡萄球菌分离株。
