Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Instituto Nacional da Propriedade Industrial, Rio de Janeiro, Brazil.
World J Microbiol Biotechnol. 2022 Nov 8;39(1):5. doi: 10.1007/s11274-022-03443-w.
Aureocin A53 is an N-formylated antimicrobial peptide (AMP) produced by Staphylococcus aureus. Aureocin A53 has a broad spectrum of antimicrobial activity against human and animal pathogens. In the present study, its antagonistic activity was investigated towards 30 strains of S. aureus and 30 strains of Streptococcus spp. isolated from bovine mastitis cases in Brazil. Bovine mastitis is a disease that causes a major economic impact worldwide. Aureocin A53 inhibited the growth of all 60 strains tested, including multidrug-resistant streptococcal isolates and strains of S. aureus belonging to different pulsotypes. This AMP proved to be bactericidal against the six target strains randomly selected among staphylococci and streptococci, also exhibiting a lytic mode of action against the staphylococcal cells. Furthermore, it was determined that 2,048 AU/mL of the AMP were required to inhibit 99.99% of the cell growth of the strain less sensitive to aureocin A53. Aureocin A53 was not toxic to bovine mammary gland epithelial cells after a 24-h exposure and maintained its antimicrobial activity when tested in the excised-teat model against strains of S. aureus and Streptococcus agalactiae, the species responsible for most intramammary infections, not only in Brazil but in other countries as well. Therefore, the use of aureocin A53 in the development of new pharmacological products for the prophylaxis and/or treatment of bovine mastitis was considered promising.
Aureocin A53 是一种由金黄色葡萄球菌产生的 N-甲酰基抗菌肽 (AMP)。Aureocin A53 对人类和动物病原体具有广谱的抗菌活性。在本研究中，研究了其对从巴西奶牛乳腺炎病例中分离的 30 株金黄色葡萄球菌和 30 株链球菌的拮抗活性。奶牛乳腺炎是一种在全球范围内造成重大经济影响的疾病。Aureocin A53 抑制了所有 60 株受试菌株的生长，包括多药耐药性链球菌分离株和属于不同脉冲型的金黄色葡萄球菌菌株。这种 AMP 被证明对随机选择的六种金黄色葡萄球菌和链球菌具有杀菌作用，对金黄色葡萄球菌细胞也表现出裂解作用。此外，确定该 AMP 的 2,048 AU/mL 可抑制对 aureocin A53 敏感性较低的菌株 99.99%的细胞生长。在 24 小时暴露后，Aureocin A53 对牛乳腺上皮细胞没有毒性，并且在切除乳头模型中对金黄色葡萄球菌和无乳链球菌（引起大多数乳腺炎感染的物种）的测试中保持其抗菌活性，不仅在巴西，而且在其他国家也是如此。因此，考虑使用 aureocin A53 开发用于预防和/或治疗奶牛乳腺炎的新型药理产品具有广阔的前景。
