人胰岛中胃蛋白酶原C的显示
Demonstration of pepsinogen C in human pancreatic islets.
作者信息
Szecsi P B, Halgreen H, Poulsen S S, Axelsson C K, Damkjaer-Nielsen M, Kjaer T, Foltmann B
机构信息
Department of Clinical Chemistry, Hvidovre Hospital, Denmark.
出版信息
Gut. 1987 Oct;28(10):1208-14. doi: 10.1136/gut.28.10.1208.
Pancreatic tissue from 16 post mortem kidney donors have been examined for the content of pepsinogens. A zymogen with electrophoretic mobility, isoelectric point and molecular weight equal to that of pepsinogen C of gastric origin was found in all specimens. A comparison between pepsinogen C extracted from pancreatic tissue and gastric mucosa demonstrated immunological identity. Quantitative measurements with a radioimmunoassay showed pepsinogen C concentrations in pancreatic tissue three to 80 times higher than those of blood serum. Immunohistochemical staining gave positive reaction for pepsinogen C only in the alpha cells of the pancreatic islets.
对16名死后肾脏捐赠者的胰腺组织进行了胃蛋白酶原含量检测。在所有标本中均发现一种酶原，其电泳迁移率、等电点和分子量与胃源性胃蛋白酶原C相同。对从胰腺组织和胃黏膜中提取的胃蛋白酶原C进行比较，结果显示二者具有免疫同一性。放射免疫分析法的定量测量结果表明，胰腺组织中胃蛋白酶原C的浓度比血清中的高3至80倍。免疫组织化学染色显示，仅在胰岛的α细胞中胃蛋白酶原C呈阳性反应。
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