Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia.
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya str. 3, 630090, Novosibirsk, Russia.
J Fluoresc. 2021 Jan;31(1):11-16. doi: 10.1007/s10895-020-02643-7. Epub 2020 Nov 6.
Light-activatable nitric oxide (NO) donors have become of interest in the recent years. They produce NO when illuminated by light, which enables the control of its local concentration and is promising for biomedical applications. Several successful prototypes of photodonors have been published, but further research is needed to improve their properties such as water-solubility, activation wavelength, biocompatibility etc. One of major challenges on this way is to evaluate the efficiency of NO generation. Several methods may be used to track NO, including spin traps, specific electrodes and fluorescence-based probes. We have studied the applicability of well-known fluorescent reporter, diaminorhodamine (DAR-2), for the evaluation of NO production by photodonors. Our results indicate that DAR-2 can be used for the quantification of NO photorelease if this process is not accompanied by the singlet oxygen formation. Otherwise the oxidation of probe results in huge fluorescence increase, which interferes with signal due to reaction with NO. This issue should be taken into account when studying hybrids releasing both NO and O, which are promising for photodynamic therapy.
近年来，光激活型一氧化氮（NO）供体受到了广泛关注。当用光照射时，它们会产生 NO，这使得可以控制其局部浓度，因此在生物医学应用中具有广阔的前景。已经发表了几种成功的光供体原型，但仍需要进一步研究来改善其性质，如水溶性、激活波长、生物相容性等。在这条道路上的主要挑战之一是评估 NO 生成的效率。有几种方法可用于跟踪 NO，包括自旋捕获剂、特定电极和基于荧光的探针。我们研究了著名的荧光报告分子二氨基罗丹明（DAR-2）在评估光供体产生 NO 方面的适用性。我们的结果表明，如果光释放过程不伴随单线态氧的形成，DAR-2 可用于 NO 光释放的定量。否则，探针的氧化会导致荧光强度大幅增加，这会干扰由于与 NO 反应而产生的信号。在研究同时释放 NO 和 O 的混合体系时，应考虑到这一问题，因为这些混合体系在光动力治疗中具有广阔的应用前景。
