检测慢性髓性白血病患者的 BCR-ABL 融合基因及其转录变体 - 一项多比较研究。

Detection of BCR-ABL fusion gene and its transcript variants in chronic myeloid leukaemia patients - a multi-comparison study.

机构信息

Government College University, Lahore, Pakistan.

King Edward Medical University, Lahore, Pakistan.

出版信息

J Pak Med Assoc. 2020 Oct;70(10):1748-11752. doi: 10.5455/JPMA.30606.

DOI:10.5455/JPMA.30606

PMID:33159746

Abstract

OBJECTIVE

To compare blood cells and plasma for BCR-ABL quantification and to find out the frequency of b2a2, b3a2 and e1a2 transcripts in chronic myeloid leukaemia patients, and to assess the correlation of BCR-ABL transcripts with haematological counts, age and gender.

METHODS

The study was conducted in April 2018 at King Edward Medical University, Lahore, Pakistan, and comprised chronic myeloid leukaemia patients from Mayo Hospital, Lahore. Ribonucleic acid was extracted using commercial extraction kits and detection of BCR-ABL messenger ribonucleic acid and its transcript variants was done by real time polymerase chain reaction. Data was analysed using SPSS 11.5.

RESULTS

Of the 48 patients, fusion of b3a2 was detected in 32(66.66%) and b2a2 in 10(32.10%), while fusion e1a2 was not detected at all. No co-expression of transcripts was seen in any patient. No significant correlation was found between transcript type and any of haematological parameters (p>0.05). No significant correlation of transcript type with gender and age was found (p>0.05). BCR-ABL/G6PD ratios in peripheral blood cells were higher than that of plasma (p<0.05).

CONCLUSIONS

Plasma can be used as an alternative to blood cells for BCR-ABL quantification, and transcript types cannot be easily explained by clinical factors.

摘要

目的

比较血液细胞和血浆用于 BCR-ABL 定量，并确定慢性髓性白血病患者中 b2a2、b3a2 和 e1a2 转录本的频率，并评估 BCR-ABL 转录本与血液学计数、年龄和性别之间的相关性。

方法

该研究于 2018 年 4 月在巴基斯坦拉合尔的爱德华国王医科大学进行，纳入了来自拉合尔梅奥医院的慢性髓性白血病患者。使用商业提取试剂盒提取核糖核酸，并通过实时聚合酶链反应检测 BCR-ABL 信使核糖核酸及其转录变体。使用 SPSS 11.5 分析数据。

结果

在 48 例患者中，检测到 32 例（66.66%）融合 b3a2 和 10 例（32.10%）融合 b2a2，而融合 e1a2 则完全未检测到。在任何患者中均未观察到转录本的共表达。未发现转录本类型与任何血液学参数之间存在显著相关性（p>0.05）。未发现转录本类型与性别和年龄之间存在显著相关性（p>0.05）。外周血细胞中的 BCR-ABL/G6PD 比值高于血浆（p<0.05）。