光诱导脉冲偶极相关光谱法中光可激发自旋标记的定点连接。
Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy.
机构信息
Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany.
出版信息
Chem Commun (Camb). 2020 Dec 4;56(93):14669-14672. doi: 10.1039/d0cc03101a. Epub 2020 Nov 7.
PMID:33159780
Abstract
Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD) spectroscopy in proteins that lack an intrinsic photoexcitable center.
摘要
光致三重态作为脉冲电子顺磁共振（EPR）光谱中的自旋标记物正日益受到关注。在这里，我们证明了荧光染料曙红 Y、孟加拉玫瑰红和 Atto Thio12 是缺乏内在光激发中心的蛋白质中通过激光诱导磁偶极（LaserIMD）光谱进行距离测定的合适标记物。
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