Harris C C
Laboratory of Human Carcinogenesis, National Cancer Institute, Bethesda, MD 20892.
IARC Sci Publ. 1987(84):20-5.
In-vitro models using human tissues and cells provide a bridge between studies of humans and of laboratory animals. Cultured human cells activate N-nitrosamines to DNA-damaging metabolites, including alkyldiazonium ions and aldehydes; these metabolites can also inactivate DNA repair processes, such as O6-alkylguanine DNA-alkyltransferase (AAT) activity, and are mutagenic in human cells. Both human epithelial and mesenchymal cells have been transformed in vitro by N-nitroso compounds.
