Curtiss R, Kelly S M
Department of Biology, Washington University, St. Louis, Missouri 63130.
Infect Immun. 1987 Dec;55(12):3035-43. doi: 10.1128/iai.55.12.3035-3043.1987.
Salmonella typhimurium SR-11 mutants with cya::Tn10 or crp::Tn10 mutations were found to be avirulent and immunogenic for BALB/c mice. Fusaric acid-resistant derivatives with deletions of the Tn10 and adjacent DNA sequences were constructed in S. typhimurium SR-11 strains with or without the virulence plasmid pStSR100. These delta cya delta crp strains grew more slowly than wild-type strains. They possessed wild-type ability to attach to, invade, and persist in gut-associated lymphoid tissue for up to a week but exhibited a diminished ability to reach mesenteric lymph nodes and the spleen. Mice 4 to 8 weeks old were resistant to oral infection with 10(9) cells of several different delta cya and delta cya delta crp strains (the equivalent to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11) and 30 days after immunization became resistant to oral challenge with 10(3) to 10(4) 50% lethal doses of wild-type S. typhimurium SR-11.
携带cya::Tn10或crp::Tn10突变的鼠伤寒沙门氏菌SR-11突变体对BALB/c小鼠无致病性但具有免疫原性。在带有或不带有毒力质粒pStSR100的鼠伤寒沙门氏菌SR-11菌株中构建了缺失Tn10和相邻DNA序列的抗镰刀菌酸衍生物。这些缺失cya和crp的菌株生长速度比野生型菌株慢。它们具有与野生型相同的附着、侵入和在肠道相关淋巴组织中持续存在长达一周的能力，但到达肠系膜淋巴结和脾脏的能力有所减弱。4至8周龄的小鼠对几种不同的缺失cya和缺失cya且缺失crp的菌株的10⁹个细胞口服感染具有抗性（相当于10⁴个野生型鼠伤寒沙门氏菌SR-11半数致死剂量），免疫30天后对10³至10⁴个野生型鼠伤寒沙门氏菌SR-11半数致死剂量的口服攻击具有抗性。
