Momburg F, Herrmann B, Moldenhauer G, Möller P
Institute of Pathology, University of Heidelberg, FRG.
Int J Cancer. 1987 Nov 15;40(5):598-603. doi: 10.1002/ijc.2910400504.
The expression of HLA-DR, HLA-DP and HLA-DQ antigens was studied in an unselected series of 66 B-cell lymphomas by means of immunohistology using monoclonal antibodies against non-polymorphic determinants in a sensitive immunoperoxidase technique. In addition, the expression of the MHC class-II antigen-associated invariant chain (li) was examined. The tumors were classified according to the Kiel classification, 30 being of high-grade and 36 being of low-grade malignancy. Only 6 lymphomas of high-grade and 16 lymphomas of low-grade malignancy showed coordinate strong expression of all HLA class-II antigens and invariant chain as observed in the normal peripheral B cell. Six further tumors of high-grade and 8 tumors of low-grade malignancy contained tumor-cell subsets with reduced expression of one or several of the antigens. Eighteen lymphomas of high-grade and 12 lymphomas of low-grade malignancy contained varying tumor-cell subsets that were negative for HLA-DR, -DP, -DQ and li in a selective or combined manner. Three highly malignant tumors were devoid of all class-II antigens and li; 2 highly malignant tumors expressed invariant chain only. The presence of high-grade malignancy was significantly correlated with the occurrence of tumor cells lacking HLA-DR (p = 0.004), HLA-DP (p = 0.013), HLA-DQ (p = 0.007) or li (p = 0.024).
采用敏感的免疫过氧化物酶技术，运用针对非多态性决定簇的单克隆抗体，通过免疫组织学方法，对66例未经选择的B细胞淋巴瘤系列进行了HLA - DR、HLA - DP和HLA - DQ抗原表达的研究。此外，还检测了MHCⅡ类抗原相关恒定链（li）的表达。肿瘤根据 Kiel 分类法进行分类，其中30例为高级别恶性肿瘤，36例为低级别恶性肿瘤。只有6例高级别淋巴瘤和16例低级别恶性淋巴瘤表现出如正常外周B细胞中观察到的所有HLAⅡ类抗原和恒定链的协同强表达。另外6例高级别肿瘤和8例低级别恶性肿瘤含有一个或几个抗原表达降低的肿瘤细胞亚群。18例高级别淋巴瘤和12例低级别恶性淋巴瘤含有以选择性或联合方式对HLA - DR、- DP、- DQ和li呈阴性的不同肿瘤细胞亚群。3例高度恶性肿瘤缺乏所有Ⅱ类抗原和恒定链；2例高度恶性肿瘤仅表达恒定链。高级别恶性肿瘤的存在与缺乏HLA - DR（p = 0.004）、HLA - DP（p = 0.013）、HLA - DQ（p = 0.007）或恒定链（p = 0.024）的肿瘤细胞的出现显著相关。
