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通过牛血清白蛋白和褐藻胶之间的美拉德反应形成含有叶黄素和玉米黄质的新型涂层材料。

Formation of a novel coating material containing lutein and zeaxanthin via a Maillard reaction between bovine serum albumin and fucoidan.

机构信息

Department of Food & Nutrition, College of Human Ecology, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 04763, Republic of Korea.

Department of Food & Nutrition, College of Human Ecology, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 04763, Republic of Korea.

出版信息

Food Chem. 2021 May 1;343:128437. doi: 10.1016/j.foodchem.2020.128437. Epub 2020 Oct 28.

DOI:10.1016/j.foodchem.2020.128437
PMID:33162255
Abstract

The effective delivery of bioactive compounds has recently been receiving attention. In this study, a conjugate with BSA and fucoidan synthesized via the Maillard reaction was confirmed through electrophoresis, the o-phthalaldehyde assay, and through changes in absorbance. Two moles of fucoidan were glycated with one mole of BSA at 60 °C and 79% relative humidity for 4 days. The droplet coated with B-F conjugate remained stable during storage at 4 and 25 °C and slightly increased only at 55 °C however, the droplet coated with intact BSA and B/F mixture significantly increased. L/Z were degraded about 82, 79, and 36% for 4, 25, and 55 °C, respectively, regardless of the type of emulsifier. Although the conjugates could not prevent the degradation of lutein and zeaxanthin during storage, they improved the stability of the emulsion and showed 4.20-fold and 1.32-fold higher bioaccessibility than intact BSA and B/F mixtures, respectively.

摘要

生物活性化合物的有效传递最近受到了关注。在这项研究中,通过电泳、邻苯二甲醛法和吸光度变化,证实了通过美拉德反应合成的与 BSA 和褐藻胶结合的缀合物。在 60°C 和 79%相对湿度下,4 天内,2 摩尔褐藻胶与 1 摩尔 BSA 糖化。在 4°C 和 25°C 下储存时,涂有 B-F 缀合物的液滴保持稳定,仅在 55°C 下略有增加,然而,涂有完整 BSA 和 B/F 混合物的液滴显著增加。L/Z 在 4°C、25°C 和 55°C 下的降解率分别约为 82%、79%和 36%,与乳化剂的类型无关。尽管缀合物不能防止叶黄素和玉米黄质在储存过程中的降解,但它们提高了乳液的稳定性,与完整的 BSA 和 B/F 混合物相比,生物利用度分别提高了 4.20 倍和 1.32 倍。

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