Hematology and Bone marrow transplantation department, Pierre and Marie Curie Center, Algiers, Algeria.
Hematology and Bone marrow transplantation department, Pierre and Marie Curie Center, Algiers, Algeria.
Transfus Apher Sci. 2020 Dec;59(6):102987. doi: 10.1016/j.transci.2020.102987. Epub 2020 Nov 2.
Peripheral blood stem cells (PBSC) are the source of allogeneic hematopoietic stem cell transplants currently used for malignant and non-malignant hematological diseases. PBSC harvest may be difficult in young children who are donors. Extracorporeal separator line priming by red blood cells or albumin alone is usually required to improve haemodynamic tolerance and efficacy of collection. We present our experience with 29 children weighing 20 kg or less mobilised between January 2005 and June 2018. The median age and weight at the time of apheresis were 5 years and 18 kg, respectively. A total of 54 PBSC were performed. The median cell yield per apheresis was 5.9 × 10 CD34cells/kg (2,5-13,9) recipient body weight (RBW). Despite their low weight, insertion of a femoral catheter was avoided in 58.6% of children. Nineteen donors required 2 or 3 apheresis sessions without any major complication. Twenty-nine pts with hemopathies have successfully benefited from PBSC except one case of rejection with aplastic anemia.
外周血干细胞（PBSC）是目前用于治疗恶性和非恶性血液病的同种异体造血干细胞移植的来源。对于作为供体的年幼儿童，可能难以采集 PBSC。通常需要单独用红细胞或白蛋白对外周血细胞分离器管路进行预充注，以提高血液动力学耐受性和采集效果。我们介绍了我们在 2005 年 1 月至 2018 年 6 月期间动员的 29 名体重 20kg 或以下的儿童的经验。在采集时的中位年龄和体重分别为 5 岁和 18kg。共进行了 54 次 PBSC 采集。每次采集的中位细胞产量为每公斤体重 5.9×10 个 CD34 细胞（2.5-13.9）。尽管体重较低，但 58.6%的儿童避免了股静脉置管。19 名供者需要进行 2 或 3 次采集，没有任何重大并发症。除 1 例再生障碍性贫血排斥反应外，29 例血液系统疾病患者成功受益于 PBSC。
