Conway de Macario E, Jovell R J, Macario A J
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany.
J Immunoassay. 1987;8(4):283-95. doi: 10.1080/15321818708057028.
Modifications to the slide immunoenzymatic assay (SIA) using the PAP reagent (SIA-PAP) were developed which increase sensitivity considerably. These SIA modifications are particularly useful for measuring speedily antibodies in dilute samples available only in microliter-volumes when antigen is scarce, whether a molecule, or a prokaryotic or eukaryotic cell.
利用PAP试剂对玻片免疫酶测定法(SIA)进行了改进(SIA-PAP),大大提高了检测灵敏度。这些SIA改进方法对于在抗原稀缺时快速检测仅以微升体积存在的稀释样品中的抗体特别有用,无论抗原是分子、原核细胞还是真核细胞。