通过用抗IgG Fc免疫球蛋白预处理来改善微量滴定板的抗体结合特性。

Improvement in the antibody binding characteristics of microtitre wells by pretreatment with anti-IgG Fc immunoglobulin.

作者信息

Sankolli G M, Stott R A, Kricka L J

机构信息

Department of Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Medical Centre, Birmingham, U.K.

出版信息

J Immunol Methods. 1987 Nov 23;104(1-2):191-4. doi: 10.1016/0022-1759(87)90503-5.

DOI:10.1016/0022-1759(87)90503-5

PMID:3316392

Abstract

A preliminary coating of the surface of polystyrene microtitre wells with anti-IgG Fc immunoglobulin improves the binding capacity of the wells for antibody and leads to a marked improvement in the reproducibility of immunoassays carried out in the wells. In an enhanced chemiluminescent enzyme immunoassay for oestradiol the binding capacity for oestradiol of wells precoated with anti-IgG Fc immunoglobulin and then coated with anti-oestradiol immunoglobulin was more than twice that of wells coated directly with anti-oestradiol immunoglobulin. Intra-assay precision in the precoated wells ranged from 1.3 to 4.0% compared to 2.9 to 6.7% (0-3200 fmol oestradiol) in wells which had not been precoated.

摘要

用抗IgG Fc免疫球蛋白对聚苯乙烯微量滴定孔表面进行预包被，可提高孔对抗体的结合能力，并显著改善在这些孔中进行的免疫测定的重现性。在一种用于雌二醇的增强化学发光酶免疫测定中，先用抗IgG Fc免疫球蛋白预包被，然后再用抗雌二醇免疫球蛋白包被的孔对雌二醇的结合能力，是直接用抗雌二醇免疫球蛋白包被的孔的两倍多。预包被孔的批内精密度范围为1.3%至4.0%，而未预包被的孔的批内精密度为2.9%至6.7%（0 - 3200 fmol雌二醇）。