一种用于检测透明质酸酶的间接酶免疫测定法。

An indirect enzymoimmunological assay for hyaluronidase.

作者信息

Delpech B, Bertrand P, Chauzy C

机构信息

Laboratory of Immunochemistry, Centre Henri-Becquerel, Rouen, France.

出版信息

J Immunol Methods. 1987 Nov 23;104(1-2):223-9. doi: 10.1016/0022-1759(87)90508-4.

DOI:10.1016/0022-1759(87)90508-4

PMID:3316396

Abstract

The use of a hyaluronic acid-binding proteoglycan (hyaluronectin) as a probe for the detection of hyaluronic acid has facilitated the development of an indirect enzymo-immunological assay for hyaluronidase. Plastic microtest ELISA plates were coated with hyaluronic acid. Incubation with hyaluronidase led to the destruction of insolubilized hyaluronic acid in proportion to the hyaluronidase concentration of samples. Residual hyaluronic acid was assayed by its capacity to bind immune complexes made up of hyaluronectin supplemented with alkaline phosphatase-conjugated anti-hyaluronectin antibodies. The technique was very sensitive and permitted the detection of as little as 10(-10) NFU of bovine testicular hyaluronidase. Hyaluronidase was detected by this technique in human sera, bee venom and culture medium of human hepatoma cell lines.

摘要

使用一种透明质酸结合蛋白聚糖（透明质连接素）作为检测透明质酸的探针，推动了一种用于检测透明质酸酶的间接酶免疫测定法的发展。塑料微量酶联免疫吸附测定（ELISA）板用透明质酸包被。与透明质酸酶一起孵育会导致固定化透明质酸按样品中透明质酸酶浓度的比例被破坏。通过其结合由补充了碱性磷酸酶偶联抗透明质连接素抗体的透明质连接素组成的免疫复合物的能力来测定残留的透明质酸。该技术非常灵敏，能够检测低至10^(-10)牛睾丸透明质酸酶纳氏荧光单位（NFU）。通过该技术在人血清、蜂毒和人肝癌细胞系培养基中检测到了透明质酸酶。