Koga K, Abe S, Hashimoto H, Yamaguchi M
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
J Immunol Methods. 1987 Dec 4;105(1):15-21. doi: 10.1016/0022-1759(87)90409-1.
Many investigators report anti-muscle antibodies using various kinds of methods. The Western-blotting method, however, has not previously been used for this purpose. We have detected antibodies to muscle contractile proteins in sera from patients with collagen disease and muscular dystrophy by this method. The antigens detected included myosin heavy and light chains, tropomyosin and troponin complex. Our method is a quick and sensitive way to determine which are the antigenic muscle contractile proteins.
许多研究者使用各种方法报告了抗肌肉抗体。然而，蛋白质免疫印迹法此前尚未用于此目的。我们已通过此方法在胶原病和肌肉营养不良患者的血清中检测到针对肌肉收缩蛋白的抗体。检测到的抗原包括肌球蛋白重链和轻链、原肌球蛋白和肌钙蛋白复合物。我们的方法是确定哪些是抗原性肌肉收缩蛋白的一种快速且灵敏的方式。
