采用固相免疫酶技术对克氏锥虫特异性抗体分泌细胞进行计数。

Enumeration of Trypanosoma cruzi-specific antibody-secreting cells by solid-phase immunoenzymatic technique.

作者信息

Caulada Z, Abrahamsohn I A

机构信息

Departamento de Imunologia, Universidade de São Paulo, Brasil.

出版信息

J Immunol Methods. 1987 Dec 4;105(1):87-95. doi: 10.1016/0022-1759(87)90417-0.

DOI:10.1016/0022-1759(87)90417-0

PMID:3316408

Abstract

A specific and sensitive enzyme-linked immunoassay is applied for enumeration of cells secreting specific antibodies to Trypanosoma cruzi antigens. Spleen cells from immunized mice are incubated in antigen-coated plastic tissue culture plates. Individual antibody-producing cells secrete antibody which binds to the antigen at close vicinity of the cells. The areas of bound antibodies are demonstrated by an enzyme-linked antibody assay as dark round spots, which can be easily enumerated. This assay allows the enumeration of specific antibody-secreting cells to parasite antigens, overcoming the failures to develop conventional plaque-forming cells assays to complex antigens.

摘要

一种特异性和敏感性高的酶联免疫测定法用于对分泌针对克氏锥虫抗原的特异性抗体的细胞进行计数。将免疫小鼠的脾细胞在包被有抗原的塑料组织培养板中孵育。单个产生抗体的细胞分泌的抗体在细胞附近与抗原结合。通过酶联抗体测定法将结合抗体的区域显示为深色圆点，易于计数。该测定法能够对针对寄生虫抗原的特异性抗体分泌细胞进行计数，克服了针对复杂抗原开发传统噬斑形成细胞测定法时遇到的困难。