Xiao-Hu Liu, Zhi-Hui Zhao, Yue Zhou, Cui-Li Wang, Yan-Jun Han, Wen Zhou
Department of Histology and Embryology, School of Basic Medical Sciences, Dali University Dali 671000, China.
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(19):4699-4704. doi: 10.19540/j.cnki.cjcmm.20200427.403.
To explore the effect of slient mating type information regulation 2 homolog 1(SIRT1) on the delay of D-galactose(D-gal) induced premature ovarian failure in mice with ginsenoside Rg_1(Rg_1). Fifty-four female SPF BALB/c mice were randomly divided into PBS group, D-gal group, and Rg_1 group. In the D-gal group, D-galactose(200 mg·kg(-1)·d(-1)) was injected subcutaneously into the neck and back for 42 days. In the PBS group, the equal amount of phosphate buffered saline(PBS) was injected into the neck and back for 42 days. In addition to the therapy of D-gal group, Rg_1 group was given Rg_1(20 mg·kg(-1)·d(-1)) through the intraperitoneal injection on the 15 th day for 28 days. At the same time, the D-gal group and the PBS group were also given an equal amount of PBS through hintraperitoneal injection on the 15 th day for 28 days. The changes in body weight and ovarian weight coefficient of mice were detected. Expressions of estradiol 2(E_2), luteinizing hormone(LH), superoxide dismutase(SOD), catalase(CAT) and follicle-stimulating hormone(FSH) in peripheral blood were detected. Morphological changes of ovaries were detected by HE staining. Changes in expression of aging regulator SIRT1 were detected by fluorescent quantitative PCR and Western blot. The results showed that compared with the PBS group, the body weight growth rate of the D-gal group was significantly slowed, the ovarian weight coefficient was decreased, the serum levels of E_2, LH, SOD, CAT were significantly reduced, and FSH was significantly increased. After the administration with Rg_1, the body weight growth rate, ovarian weight coefficient, serum levels of E_2, LH, SOD, and CAT in the mice were higher than those in the D-gal group, while FSH was lower than those in the D-gal group. HE staining showed that the follicular morphology and structure of the PBS group were normal; the number of follicles in the D-gal group was reduced, the corpus luteum was vacuolated, and the number of atretic follicles was increased. Compared with the D-gal group, the number of follicles in the Rg_1 group was increased, whereas the number of corpus luteum was decreased. Compared with the PBS group, SIRT1 expression was down-regulated in the D-gal group, while SIRT1 expression was up-regulated in the Rg_1 group compared with the D-gal group. The results suggested that Rg_1 could delay D-gal-induced premature ovarian failure in a mouse model of premature ovarian failure, and SIRT1 played an important role in this.
探讨沉默信息调节因子2同源物1（SIRT1）在人参皂苷Rg_1（Rg_1）延缓D-半乳糖（D-gal）诱导的小鼠卵巢早衰中的作用。将54只雌性SPF级BALB/c小鼠随机分为PBS组、D-gal组和Rg_1组。D-gal组小鼠颈部和背部皮下注射D-半乳糖（200 mg·kg⁻¹·d⁻¹），连续注射42天。PBS组小鼠颈部和背部皮下注射等量的磷酸盐缓冲液（PBS），连续注射42天。Rg_1组在第15天除给予D-gal组相同处理外，腹腔注射Rg_1（20 mg·kg⁻¹·d⁻¹），持续28天。同时，D-gal组和PBS组在第15天也腹腔注射等量PBS，持续28天。检测小鼠体重及卵巢重量系数的变化。检测外周血中雌二醇2（E₂）、黄体生成素（LH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和卵泡刺激素（FSH）的表达。通过HE染色检测卵巢的形态学变化。通过荧光定量PCR和蛋白质免疫印迹法检测衰老调节因子SIRT1表达的变化。结果显示，与PBS组相比，D-gal组小鼠体重增长率显著减慢，卵巢重量系数降低，血清E₂、LH、SOD、CAT水平显著降低，FSH水平显著升高。给予Rg_1后，小鼠体重增长率、卵巢重量系数、血清E₂、LH、SOD和CAT水平均高于D-gal组，而FSH水平低于D-gal组。HE染色显示，PBS组卵泡形态和结构正常；D-gal组卵泡数量减少，黄体空泡化，闭锁卵泡数量增加。与D-gal组相比，Rg_1组卵泡数量增加，黄体数量减少。与PBS组相比，D-gal组SIRT1表达下调，而与D-gal组相比，Rg_1组SIRT1表达上调。结果提示，Rg_1可延缓D-gal诱导的小鼠卵巢早衰模型中卵巢早衰的发生，且SIRT1在此过程中发挥重要作用。
