Chen Di, Guo Yongchun, Chen Xuejin, Wang Pengjie, Chen Guixin, Ye Naixing
Key Laboratory of Tea Science at Universities in Fujian, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
Sheng Wu Gong Cheng Xue Bao. 2020 Sep 25;36(9):1869-1886. doi: 10.13345/j.cjb.190579.
Jasminum sambac is a perennial evergreen shrub plant. With fragrant and aroma, and often used as a raw material for natural spices. In this study, we used white light as the control group, red-light and blue-light as the treatment to study effects of different light on jasmine flowering. Red- light promoted jasmine flowering in advance and increased the number of flower buds, whereas blue-light delayed jasmine flowering and decreased the number of flower buds. There was significant difference on the number of flower buds among the three groups. The top buds' transcriptomes of different light were sequenced by the Illumina Hiseq/Miseq 2000 high-throughput sequencing technology. In total 2 452 457 Unigenes were generated by transcriptome sequencing, of which 1 760 723 Unigenes were annotated into GO, COG, KEGG, KOG, NR, Pfam, Swiss-Prot, NOG databases. There were 894 DEGs in the control group vs red-light group, 2 690 DEGs in the control group vs blue-light group, and 3 828 DEGs in the red-light group vs blue-light group. KEGG Enrichment analysis reveals that the significant enrichment pathways had 6 pathways, including secondary metabolite biosynthesis, phenylpropanoid biosynthesis, indole alkaloid biosynthesis, photosynthesis, plant hormone signaling, and plants-pathogen interactions, and 24 related DEGs were detected by RT-qPCR, the result of which was significantly correlated with the transcriptome data. Through further analysis of transcriptome data, a large number of flowering-related hormones (IAA, ETH, GA, CTK, ABA, SA, JA) signal transduction genes and flowering pathway-related regulatory genes (PHY, CRY1, FPA, AGL and SOC1) and transcription factor (bHLH, MYB, WKRY) family genes were found. The study will help elucidate the differential expression mechanism of different light regulation of jasmine flowering.
茉莉是一种多年生常绿灌木植物。其具有芬芳香气，常被用作天然香料的原料。在本研究中，我们以白光为对照组，红光和蓝光为处理组，研究不同光照对茉莉开花的影响。红光促进茉莉提前开花并增加花芽数量，而蓝光延迟茉莉开花并减少花芽数量。三组之间花芽数量存在显著差异。采用Illumina Hiseq/Miseq 2000高通量测序技术对不同光照下的顶芽转录组进行测序。转录组测序共产生2452457个单基因，其中1760723个单基因被注释到GO、COG、KEGG、KOG、NR、Pfam、Swiss-Prot、NOG数据库中。对照组与红光组之间有894个差异表达基因（DEG），对照组与蓝光组之间有2690个DEG，红光组与蓝光组之间有3828个DEG。KEGG富集分析显示，显著富集的通路有6条，包括次生代谢物生物合成、苯丙烷生物合成、吲哚生物碱生物合成、光合作用、植物激素信号传导和植物-病原体相互作用，通过RT-qPCR检测到24个相关DEG，其结果与转录组数据显著相关。通过对转录组数据的进一步分析，发现了大量与开花相关的激素（IAA、ETH、GA、CTK、ABA、SA、JA）信号转导基因以及开花途径相关的调控基因（PHY、CRY1、FPA、AGL和SOC1）和转录因子（bHLH、MYB、WKRY）家族基因。该研究将有助于阐明不同光照调控茉莉开花的差异表达机制。
