Laboratório de Educação e Evolução Prof. Horacio Schneider, Instituto de Ciências da Educação, Universidade Federal do Oeste do Pará, Santarém, Brasil.
Laboratório de Genética e Conservação, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Brasil.
Mitochondrial DNA A DNA Mapp Seq Anal. 2021 Jan;32(1):1-11. doi: 10.1080/24701394.2020.1844677. Epub 2020 Nov 9.
The mitochondrial cytochrome oxidase c subunit 1 (COI) gene has been widely used in phylogenetic studies of crustaceans and analyses in population genetics. As COI studies have become more popular, there has been an increase in the number of reports of the presence of nuclear insertions of mitochondrial DNA (Numts) and mitochondrial heteroplasmy. Here, we provide evidence of both types of event in the COI sequences of , an economically important freshwater prawn, which is widespread in South America. Heteroplasmy and Numts were confirmed by different methods of DNA extraction (genomic, mitochondrial, and nuclear-enriched DNA), cloning, and sequencing, and were observed in 11 of the 14 populations sampled, primarily in the Amazon region. We discuss how the occurrence of these events affects the interpretation of the genetic relationships among the populations, and we recommend caution when using COI for genetic inferences in prawns of the genus , and in particular that any analysis should include nuclear markers.
线粒体细胞色素氧化酶亚基 1（COI）基因已广泛应用于甲壳类动物的系统发育研究和种群遗传学分析。随着 COI 研究的普及，越来越多的报告表明存在线粒体 DNA（Numts）的核插入和线粒体异质性。在这里，我们提供了南美洲广泛分布的经济上重要的淡水虾（）的 COI 序列中这两种类型事件的证据。通过不同的 DNA 提取方法（基因组、线粒体和核富集 DNA）、克隆和测序证实了异质性和 Numts，并在 14 个采样群体中的 11 个群体中观察到，主要在亚马逊地区。我们讨论了这些事件的发生如何影响对群体间遗传关系的解释，并建议在使用 COI 进行属虾的遗传推断时要谨慎，特别是任何分析都应包括核标记。
