Juan Cristina, de Simone Gaia, Sagratini Gianni, Caprioli Giovanni, Mañes Jordi, Juan-García Ana
Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Burjassot, València, Spain.
Laboratory of Food Chemistry, School of Pharmacy, University of Camerino, Via S. Agostino 1, 62032, Camerino, Italy.
Toxicon. 2020 Dec;188:164-171. doi: 10.1016/j.toxicon.2020.10.017. Epub 2020 Oct 24.
In the present work, different natural compounds from coffee by-product extracts (coffee silverskin and spent coffee) rich in polyphenols, was investigated against beauvericin (BEA) induced-cytotoxicity on SH-SY5Y cells. Spent coffee arise as waste products through the production of instant coffee and coffee brewing; while the silverskin is a tegument which is removed and eliminated with toasting coffee grains. First of all, polyphenol extraction methods, measurement of total polyphenols content and its identification were carried out. Afterwards evaluating in vitro effects with MTT assay on SH-SY5Y cells of coffee by-product extracts and mycotoxins at different concentrations and exposure times was performed. TPC in silverskin coffee by-product extracts was >10 times higher than in spent coffee by-product extracts. Chlorogenic acid was the majority polyphenol detected. Viability for BEA reached IC values at 72h (2.5 μM); boiling water silverskin coffee extract reached the highest viability also in pre-treatment BEA exposure and compared with MeOH and MeOH:HO (v/v, 50:50) extracts. These results in SH-SY5Y cells highlight the use of such residues as supplements or bioactive compounds in the future.
在本研究中，对富含多酚的咖啡副产品提取物（咖啡银皮和咖啡渣）中的不同天然化合物进行了研究，以考察其对博韦里霉素（BEA）诱导的SH-SY5Y细胞毒性的影响。咖啡渣是速溶咖啡生产和咖啡冲泡过程中产生的废弃物；而银皮是在烘焙咖啡豆时被去除和丢弃的外皮。首先，进行了多酚提取方法、总多酚含量的测定及其鉴定。随后，采用MTT法评估了不同浓度和暴露时间下咖啡副产品提取物和霉菌毒素对SH-SY5Y细胞的体外作用。咖啡银皮副产品提取物中的总酚含量比咖啡渣副产品提取物高10倍以上。检测到的主要多酚是绿原酸。BEA在72小时时的半数抑制浓度（IC）为2.5 μM；沸水提取的咖啡银皮提取物在预处理BEA暴露时以及与甲醇和甲醇:水（v/v，50:50）提取物相比时，也达到了最高的细胞活力。这些在SH-SY5Y细胞中的结果凸显了未来将此类残渣用作补充剂或生物活性化合物的用途。
