School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Int J Phytoremediation. 2021;23(6):585-596. doi: 10.1080/15226514.2020.1842997. Epub 2020 Nov 9.
In the current study, we investigated the potential of ethylenediaminetetraacetic acid (EDTA) assisted phytoremediation potential of L. to remediate lead (Pb)-contaminated soils. The exhibited various stress tolerance mechanisms via plant growth promoting (PGP) traits, intrinsic extracellular enzyme production and antibiotic resistance. A greenhouse experiment was conducted to examine the dual effects of plant growth promoting endophytic bacteria (PGPEB)-chelator synergy in maize plants under different Pb contaminated soil regimes. -EDTA (5 mM EDTA kg) complex significantly ( < 0.05) enhanced plant growth and biomass (48.91%); chlorophyll , and carotenoid contents (27.26%, 25.02% and 42.09%); relative water content (61.33%); proline content (63.60%); root and shoot Pb accumulation capacity (52.31% and 44.71%) in Pb contaminated soils. This may suggest the efficacy of current approach in enhancing plant tolerance capability toward Pb-uptake and phytoremediation capacity. Moreover, maize plants showed differential response to Pb availability in soil-1 (S1; Pb spiked soil, 500 mg kg) and soil-2 (S2; aged-contaminated soil) under various treatments. We describe the intriguing role of -EDTA-maize system for Pb decontamination which can be used as a base line to explore the proposed combinatorial approach for long-term trails under field conditions for reclamation of Pb-contaminated soils.HighlightsThe PGPEB-EDTA mediated potential of against Pb spiked and industrial contaminated soils is noticed.Increased tolerance of against Pb in association with , and EDTA is reported first time.Enhanced accumulation of metals by is reported under combined treatment of , and EDTA.Inoculation of plants with , and EDTA has positive effects on growth and accumulation of Pb by .
在当前的研究中，我们调查了乙二胺四乙酸（EDTA）辅助植物修复的潜力，以修复受铅（Pb）污染的土壤。该植物通过植物生长促进（PGP）特性、内在细胞外酶的产生和抗生素抗性表现出各种胁迫耐受机制。进行了一项温室实验，以研究不同 Pb 污染土壤条件下，植物生长促进内生细菌（PGPEB）-螯合剂协同作用对玉米植株的双重影响。-EDTA（5mM EDTA kg）络合物显著（<0.05）提高了植物生长和生物量（48.91%）；叶绿素、类胡萝卜素和类胡萝卜素含量（27.26%、25.02%和 42.09%）；相对含水量（61.33%）；脯氨酸含量（63.60%）；根和茎叶中 Pb 的积累能力（52.31%和 44.71%）在 Pb 污染土壤中。这可能表明，目前的方法在提高植物对 Pb 吸收的耐受能力和植物修复能力方面是有效的。此外，玉米植株在不同处理下对土壤-1（S1；Pb 污染土壤，500mg kg）和土壤-2（S2；老化污染土壤）中 Pb 的有效性表现出不同的反应。我们描述了 -EDTA-玉米系统对 Pb 脱污染的有趣作用，这可以作为探索拟议的组合方法的基线，以便在田间条件下进行长期试验，以修复 Pb 污染土壤。要点注意到 PGPEB-EDTA 介导的对 Pb 污染土壤的潜力增加了与和 EDTA 相关的对 Pb 的耐受性，这是首次报道。在和 EDTA 的联合处理下，报告了金属的积累增强。在和 EDTA 的联合处理下，植物接种对 Pb 的生长和积累有积极影响。
