Department of Ophthalmology, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Ophthalmology, Mackay Memorial Hospital, Hsinchu, Taiwan.
College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Exp Eye Res. 2021 Jan;202:108352. doi: 10.1016/j.exer.2020.108352. Epub 2020 Nov 6.
This study investigated the effects of growth factors and internal limiting membrane components on Müller cell migration. We studied the effects of epidermal growth factor (EGF), fibroblast growth factor (FGF), somatomedin (IGF-1), platelet derived growth factor (PDGF), and stromal cell-derived factor-1 alpha (SDF-1α) as well as collagen IV, laminin, and fibronectin on the proliferative and migratory activities of rat Müller cells in vitro. A water soluble tetrazolium-1 assay was used to quantify the viability of Müller cells in respective cultures, and analysis was performed using an enzyme-linked immunosorbent assay reader. All the factors examined had significant proliferative effects on cultured Müller cells (p < .05). A two-well Ibidi silicone culture insert was used to assess Müller cell migration. Müller cells cultured in EGF, FGF, IGF-1, collagen IV, and laminin but not in SDF, PDGF, or fibronectin effectively increased the cell migratory activity (p < .001). In addition, combined EGF and collagen IV, combined FGF and collagen IV, and combined IGF-1 and laminin exhibited more significant (p < .001) effects on Müller cell migration compared with culture a single factor. In summary, this study revealed the combinatorial effects of various growth factors and individual internal limiting membrane constituents. This may assist Müller cell migration together with the macular hole healing process.
本研究探讨了生长因子和内界膜成分对 Müller 细胞迁移的影响。我们研究了表皮生长因子 (EGF)、成纤维细胞生长因子 (FGF)、somatomedin (IGF-1)、血小板衍生生长因子 (PDGF) 和基质细胞衍生因子-1α (SDF-1α) 以及胶原 IV、层粘连蛋白和纤维连接蛋白对体外培养大鼠 Müller 细胞增殖和迁移活性的影响。采用水溶性四唑盐-1 测定法定量检测各培养物中 Müller 细胞的活力，并采用酶联免疫吸附试验阅读器进行分析。所有检查的因素对培养的 Müller 细胞均有显著的增殖作用（p<.05）。使用双孔 Ibidi 硅酮培养插入物评估 Müller 细胞迁移。在 EGF、FGF、IGF-1、胶原 IV 和层粘连蛋白中培养的 Müller 细胞，但不在 SDF、PDGF 或纤维连接蛋白中培养的 Müller 细胞有效增加了细胞迁移活性（p<.001）。此外，EGF 和胶原 IV 的组合、FGF 和胶原 IV 的组合以及 IGF-1 和层粘连蛋白的组合对 Müller 细胞迁移的影响更为显著（p<.001），与单一因素培养相比。总之，本研究揭示了各种生长因子和单个内界膜成分的组合效应。这可能有助于 Müller 细胞迁移和黄斑裂孔愈合过程。
