Université de Strasbourg, CNRS, IPHC UMR 7178, Laboratoire de Reconnaissance et Procédés de Séparation Moléculaire (RePSeM), ECPM 25 rue Becquerel F-67000 Strasbourg, France.
UMR PAM, Université de Bourgogne/Agrosup Dijon, Institut Universitaire de la Vigne et du Vin - Jules Guyot, 21000 Dijon, France.
Bioresour Technol. 2021 Jan;320(Pt A):124334. doi: 10.1016/j.biortech.2020.124334. Epub 2020 Oct 29.
The aim of this work was to assess the performances of wine byproduct biomass for hydrogen production by dark fermentation. Grape must deposits from two grape varieties (Pinot Gris and Chardonnay) were considered, either with external microbial inoculum or without. We show that grape must residues contain endogenous microflora, well adapted to their environment, which can degrade sugars (initially contained in the biomass) to hydrogen without any nutrient addition. Indeed, hydrogen production during endogenous fermentation is as efficient as with an external heat-treated inoculum (2.5 ± 0.4 L.L and 1.61 ± 0.41 mol.mol, respectively) with a lower energy cost. Hydrogen-producing bacteria were selected from the endogenous microflora during semi-batch bioreactor operation, as shown by T-RFLP profiles and 16S rRNA sequencing, with Clostridium spp. (butyricum, beijerinckii, diolis, roseum) identified as the major phylotype. Such hydrogen production efficiency opens new perspectives for innovating in the valorization of winery by-products.
本工作旨在评估葡萄酒副产物生物质通过暗发酵生产氢气的性能。考虑了两种葡萄品种（灰皮诺和霞多丽）的葡萄汁渣，分别添加或不添加外部微生物接种物。我们表明，葡萄汁渣含有内源性微生物群，它们非常适应其环境，可以在不添加任何营养物质的情况下将糖（最初包含在生物质中）降解为氢气。事实上，内源发酵过程中的氢气生产效率与外部热处理接种物一样高效（分别为 2.5±0.4 L.L 和 1.61±0.41 mol.mol，而能源成本更低）。通过半分批生物反应器操作从内源性微生物群中选择了产氢细菌，如 T-RFLP 图谱和 16S rRNA 测序所示，主要的生物型是梭菌属（丁酸梭菌、贝氏梭菌、二型梭菌、玫瑰梭菌）。这种产氢效率为创新酿酒厂副产物的利用开辟了新的前景。
